LRRC45 Is a Centrosome Linker Component Required for Centrosome Cohesion
LRRC45 Is a Centrosome Linker Component Required for Centrosome Cohesion
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LRRC45 是中心体凝聚所需的中心体连接元件
DOI：
10.1016/j.celrep.2013.08.005
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发表时间：
2013-09-01
期刊：
影响因子：
8.8
通讯作者：
Chen, Jianguo
中科院分区：
文献类型：
--
作者：
He, Runsheng;Huang, Ning;Chen, Jianguo
在分裂间期，中心体通过中心粒近端之间的蛋白质连接体连接，这对于中心体作为单个微管组织中心发挥作用是重要的。然而，中心体连接子的组成和调控仍然很大程度上未知。在这里，我们表明，LRRC 45是一个中心体连接器，定位在近端的中心粒和它们之间形成纤维状结构。LRRC 45的缺失导致间期中心体分裂。此外，LRRC 45与C-Nap 1和rootaoke两者相互作用，并且在有丝分裂期间在S661处被Nek 2A磷酸化。磷酸化后，LRRC 45中心体定位和纤维样结构均显著减少，随后导致中心体分离。因此，LRRC 45是两个中心粒之间的蛋白质接头的关键组分，并且是中心体凝聚所必需的。
During interphase, centrosomes are connected by a proteinaceous linker between the proximal ends of the centrioles, which is important for the centrosomes to function as a single microtubule-organizing center. However, the composition and regulation of centrosomal linker remain largely unknown. Here, we show that LRRC45 is a centrosome linker that localizes at the proximal ends of the centrioles and forms fiber-like structures between them. Depletion of LRRC45 results in centrosome splitting during interphase. Moreover, LRRC45 interacts with both C-Nap1 and rootletin and is phosphorylated by Nek2A at S661 during mitosis. After phosphorylation, both LRRC45 centrosomal localization and fiber-like structures are significantly reduced, which subsequently leads to centrosome separation. Thus, LRRC45 is a critical component of the proteinaceous linker between two centrioles and is required for centrosome cohesion.