Detection of a cellular oncogene in spontaneous liver tumors of B6C3F1 mice.
Detection of a cellular oncogene in spontaneous liver tumors of B6C3F1 mice.
复制标题
B6C3F1 小鼠自发性肝肿瘤中细胞癌基因的检测。
作者：
T. Fox;P. Watanabe
在 24 个月大的 B6C3F1 小鼠自发产生的肝细胞肿瘤中发现了一种活跃的细胞癌基因。从肿瘤组织中分离并转染到 NIH 3T3 细胞中的 DNA 显示出 82%（11 只动物中的 9 只）的灶诱导频率。相比之下，从同一动物的周围非肿瘤肝组织中分离出的 DNA 以及来自其他 24 个月大的无肿瘤 B6C3F1 小鼠的 DNA 在 NIH 3T3 细胞测定中并未引起转化。该小鼠品系广泛用于致癌物生物测定，观察到的高转化频率（82%，而人类为 10% 至 20%）支持了 B6C3F1 小鼠对肝脏肿瘤发展高度敏感的概念。它还强调在评估潜在的人类风险之前，需要进一步了解用于长期毒性和致癌性研究的动物的致癌基因激活机制。
An active cellular oncogene was demonstrated in hepatocellular neoplasms arising spontaneously in 24-month-old B6C3F1 mice. DNA isolated from the tumorous tissue and transfected into NIH 3T3 cells showed an 82 percent (9 of 11 animals) frequency of foci induction. In contrast, DNA isolated from the surrounding nontumorous hepatic tissue from the same animals and DNA from other 24-month-old B6C3F1 mice without tumors did not cause transformation in the NIH 3T3 cell assay. This strain of mouse is used extensively in carcinogen bioassays, and the observed high frequency of transformation (82 percent, compared to 10 to 20 percent in humans) supports the concept that the B6C3F1 mouse is hypersusceptible to liver tumor development. It also emphasizes the need to further understand the mechanisms of oncogene activation in animals used for long-term studies of toxicity and oncogenicity before evaluating potential human risk.
五种人类乳腺癌细胞系之一中 c-myc 癌基因的扩增。
影响因子：
11.2
作者：
Kozbor,D;Croce,CM
通讯作者：
Croce,CM