A monoclonal antibody produced during infection which recognizes an epitope of influenza hemagglutinin only in the context of H-2k MHC class I antigen.
A monoclonal antibody produced during infection which recognizes an epitope of influenza hemagglutinin only in the context of H-2k MHC class I antigen.
复制标题
感染期间产生的单克隆抗体，仅在 H-2k MHC I 类抗原的背景下识别流感血凝素的表位。
作者：
L. McLain;N. Dimmock
从感染流感病毒（A/WSN）的H-2k小鼠肺中分离的细胞制备杂交瘤，产生单克隆IgM (LM41)，该IgM仅在H-2k细胞中表达时才能识别A/WSN血凝素（HA） Ag的表位，如Ag结合和补体介导的裂解所示。然而，它不与病毒颗粒的天然HA结合，也不与A/ wsn感染的H-2d细胞上存在的任何形式的HA蛋白结合。LM41通过a /WSN病毒感染的H-2k小鼠的CTL抑制表达a /WSN HA的重组牛苗病毒感染的H-2k靶细胞的ag特异性、MHC i类限制性裂解。我们得出结论，LM41具有与ha特异性TCR相似的Ag特异性。
A hybridoma prepared from cells isolated from the lungs of H-2k mice infected with influenza virus (A/WSN) produces a monoclonal IgM (LM41) which recognizes an epitope of the A/WSN hemagglutinin (HA) Ag only when it is expressed in H-2k cells, as shown by Ag binding and complement-mediated lysis. However, it did not bind to the native HA of virus particles or any form of HA protein present on A/WSN-infected H-2d cells. LM41 inhibited Ag-specific, MHC class I-restricted lysis of H-2k target cells infected with a vaccinia recombinant virus expressing the HA of A/WSN by CTL from A/WSN virus-infected H-2k mice. We conclude that LM41 has a similar Ag specificity to the HA-specific TCR.