Sequence and antigenic diversity in two immunodominant regions of the L2 protein of human papillomavirus types 6 and 16.
Sequence and antigenic diversity in two immunodominant regions of the L2 protein of human papillomavirus types 6 and 16.
复制标题
人乳头瘤病毒 6 型和 16 型 L2 蛋白两个免疫显性区域的序列和抗原多样性。
DOI：
10.1093/infdis/168.3.743
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Galloway,DA
中科院分区：
文献类型：
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作者：
Yaegashi,N;Xi,L;Batra,M;Galloway,DA
为了解人乳头瘤病毒(HPV)6型和16型微小衣壳蛋白(L2)的序列和抗原多样性程度，采集24份临床标本，通过聚合酶链式反应(PCR)扩增免疫优势表位6U3和16REx的编码区，测序后克隆到PATH载体中，并检测其与人血清的反应性。在11个HPV-6 DNA中，有两个与6U3区的原型毒株相同，而9个变异体在第5020位发生G到A转换，将Valine残基改变为异亮氨酸。在未与原型HPV-6L2融合蛋白反应的16份血清中，有2份与6U3-异亮氨酸变异体反应，与原型反应的8份血清也与该变异体反应。在13个HPV-16DNA中，12个与16REx区的原型毒株相同，而1个变异体在4825位发生C到G颠换，将Pro改变为精氨酸，但不影响抗原性。
To assess the extent of sequence and antigenic diversity in the minor capsid proteins (L2) of human papillomavirus (HPV) types 6 and 16, 24 clinical samples were obtained, and the regions encoding the immunodominant epitopes 6U3 and 16REx were amplified by polymerase chain reaction, sequenced, cloned into pATH plasmids, and tested for reactivity with human sera. Two of 11 HPV-6 DNAs were identical to the prototype strain in the 6U3 region, while 9 variants had a G to A transition at nt 5020, changing a valine residue to isoleucine. Of 16 sera that did not react with the prototype HPV-6 L2 fusion protein, 2 reacted with the 6U3-isoleucine variant, and all 8 sera that reacted with the prototype also reacted with the variant. Twelve of 13 HPV-16 DNAs were identical to the prototype strain in the 16REx region, while 1 variant had a C to G transversion at nt 4825, changing a proline to an arginine, but not affecting antigenicity.