Central V1b Receptor Antagonism in Lactating Rats: Impairment of Maternal Care But Not of Maternal Aggression
Central V1b Receptor Antagonism in Lactating Rats: Impairment of Maternal Care But Not of Maternal Aggression
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DOI：
10.1111/jne.12226
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发表时间：
2014-12-01
影响因子：
3.2
通讯作者：
Bosch, O. J.
中科院分区：
文献类型：
--
作者：
Bayerl, D. S.;Klampfl, S. M.;Bosch, O. J.
啮齿类动物的母性行为是由中枢催产素和加压素系统等介导的。加压素通过V1 a受体（V1 aR）作用于母亲的照顾和母亲的攻击性，其作用最近被描述。然而，V1 b受体（V1 bR）在母性行为中的潜在参与仅在基因敲除小鼠中得到证实。本研究的目的是研究哺乳期Wistar大鼠V1 bR的中枢药理学操作对母体行为的影响。在妊娠第18天，向雌性大鼠植入靶向侧脑室的引导套管。分娩后，母鼠接受了一种特异性V1 bR激动剂的急性中枢输注（d[Leu 4，Lys 8]VP）或V1 bR拮抗剂（SSR 149415）每日一次，随后观察母体护理[哺乳日（LD）1]、幼仔取回试验中的母体动机（LD 2），焦虑相关行为的高架十字迷宫（LD 3）和母亲的攻击性在母亲的防御测试，然后由产妇护理监测（LD 4）。我们的数据表明，在非应激条件下，V1 bR拮抗剂降低了发生的护理和母婴互动，而V1 bR激动剂不影响任何参数。在应激条件下（即母体防御试验后），输注V1 bR拮抗剂可降低母鼠-幼仔相互作用。在母亲的防御测试，无论是治疗影响侵略性或非侵略性的行为。最后，两种治疗都没有改变母亲的动机或焦虑。总之，中央V1 bR拮抗调节方面的产妇护理，但不是母亲的侵略或母亲的动机在哺乳期大鼠。这些发现进一步扩展了我们对加压素系统作为母体行为重要介质的认识。
Maternal behaviour in rodents is mediated by the central oxytocin and vasopressin systems, amongst others. The role of vasopressin, acting via the V1a receptor (V1aR), on maternal careand maternal aggression has recently been described. However, a potential involvement of the V1b receptor (V1bR) in maternal behaviour has only been demonstrated in knockout mice. The present study aimed to examine the effects of central pharmacological manipulation of the V1bR on maternal behaviour in lactating Wistar rats. On pregnancy day 18, female rats were implanted with a guide cannula targeting the lateral ventricle. After parturition, dams received an acute central infusion of a specific V1bR agonist (d[Leu4,Lys8]VP) or V1bR antagonist (SSR149415) once daily, followed by observations of maternal care [lactation day (LD) 1], maternal motivation in the pup retrieval test (LD 2), anxiety-related behaviour on the elevated plus-maze (LD 3) and maternal aggression in the maternal defence test followed by maternal care monitoring (LD 4). Our data demonstrate that, under nonstress conditions, the V1bR antagonist decreased the occurrence of both nursing and mother-pup interaction, whereas the V1bR agonist did not affect either parameter. Under stress conditions (i.e. after the maternal defence test), mother-pup interaction was decreased by infusion of the V1bR antagonist. During the maternal defence test, neither treatment affected aggressive or non-aggressive behaviour. Finally, neither treatment altered maternal motivation or anxiety. In conclusion, central V1bR antagonism modulates aspects of maternal care but not of maternal aggression or maternal motivation in lactating rats. These findings further extend our knowledge on the vasopressin system as a vital mediator of maternal behaviour.