Subepithelial dendritic B cells in orofacial granulomatosis.
Subepithelial dendritic B cells in orofacial granulomatosis.
复制标题
口面部肉芽肿中的上皮下树突状 B 细胞。
DOI：
10.1002/ibd.21169
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发表时间：
2010
影响因子：
4.9
通讯作者：
Patel P
中科院分区：
文献类型：
--
作者：
Patel P
面部肉芽肿病（OFG）是一种慢性，毁容，肉芽肿性炎症的嘴唇和口腔黏膜。发病机制尚不清楚，但以前曾与克罗恩病（CD）有关，最近与饮食敏感性有关。口腔黏膜是一个免疫应答部位，与保护性粘膜的产生和对疫苗接种的全身免疫应答有关，也与某些个体对过敏原的高反应有关。传统上，口腔黏膜的免疫应答被认为是由粘膜相关淋巴组织（MALT）介导的，这是与上皮密切相关的次级淋巴滤泡。方法采用免疫组化方法观察OFG及对照组织的炎症浸润情况。采用聚合酶链反应（PCR）、PCR产物克隆和测序技术对OFG的局部免疫球蛋白基因谱进行了分析。结果：我们描述了口腔粘膜中与局部上皮下微环境中任何有组织淋巴组织无关的大的、活跃的树突状B细胞。它们表达激活诱导胞苷脱氨酶，这是免疫球蛋白基因通过体细胞超突变和类开关重组实现多样化所必需的。这些B细胞也表达IgE。在形态学、增殖活性或表型方面，它们与任何其他先前描述的次级淋巴组织中的b细胞亚群不一致。结论这些上皮下树突状B细胞可能参与了口腔黏膜的免疫反应，包括ige介导的过敏反应。对于OFG患者，进一步了解这些细胞在口腔免疫中的作用可能会带来新的治疗可能性。（肠炎病2010）
BackgroundOrofacial granulomatosis (OFG) is a chronic, disfiguring, granulomatous inflammation of the lips and oral mucosa. The pathogenesis is unknown, but it has been linked previously to Crohn's disease (CD) and more recently to dietary sensitivity. The oral mucosa is an immunologically responsive site associated with the generation of protective mucosal and systemic immune responses to vaccination and also hyperresponsiveness to allergens in some individuals. Classically, immune responses in oral mucosa are considered to be mediated by mucosa-associated lymphoid tissues (MALT), secondary lymphoid follicles that are intimately associated with epithelia.MethodsImmunohistochemistry was used to investigate the inflammatory infiltrate in OFG and control tissue samples. Polymerase chain reaction (PCR), cloning of PCR products, and sequencing were used to characterize the local immunoglobulin gene profile in OFG.ResultsWe describe large, active, dendritic B cells in oral mucosa that were not associated with any organized lymphoid tissues in the local subepithelial microenvironment. They express activation induced cytidine deaminase, which is essential for immunoglobulin gene diversification by somatic hypermutation and class switch recombination. IgE is also expressed by these B cells. They do not align with any other previously described B-cell subset in secondary lymphoid tissues in terms of morphology, proliferative activity, or phenotype.ConclusionsThese subepithelial dendritic B cells may contribute to the immune responsiveness of the oral mucosa, including IgE-mediated allergic responses. In patients with OFG, further understanding of the role these cells play in oral immunity may lead to novel therapeutic possibilities. (Inflamm Bowel Dis 2010)