DNA Polymerase Required for Rapid Repair of X-ray—Induced DNA Strand Breaks in vivo
DNA Polymerase Required for Rapid Repair of X-ray—Induced DNA Strand Breaks in vivo
复制标题
体内 X 射线诱导的 DNA 链断裂快速修复所需的 DNA 聚合酶
作者：
C. Town;Kendric C. Smith;H. Kaplan
在给定剂量的x射线照射后，DNA聚合酶缺陷突变体大肠杆菌K-12 pol A1的DNA单链断裂率比以前在大肠杆菌中发现的高得多。单链断裂的增加是由于缺乏快速修复系统，这在大肠杆菌K-12中没有描述。这种缺失可能解释了pol A1突变体的x射线敏感性。pol+细胞的快速修复系统可以被可逆地抑制。
A much higher yield of DNA single-strand breaks was obtained in the DNA polymerase-deficient mutant Escherichia coli K-12 pol A1 after a given dose of x-rays than had been found before in Escherichia coli. The increased yield of single-strand breaks was due to the absence of a rapid repair system, which had not been described in Escherichia coli K-12. This absence probably accounts for the x-ray sensitivity of the pol A1 mutant. The rapid repair system can be reversibly inhibited in pol+ cells.