Survival and function of isolated hepatocytes after cryopreservation
Survival and function of isolated hepatocytes after cryopreservation
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DOI：
10.1016/s0009-2797(99)00087-3
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发表时间：
1999-06-01
影响因子：
5.1
通讯作者：
Guyomard, C
中科院分区：
文献类型：
--
作者：
Guillouzo, A;Rialland, L;Guyomard, C
液氮冷冻保存是目前长期保存分离肝细胞的唯一方法。冻融条件尚未明确；最关键的参数似乎是冷冻保护剂的选择、冷冻介质的成分以及冷却和解冻速率。来自不同物种（包括人类）的肝细胞已经获得了可比较的结果。冷冻保存通常会导致细胞恢复率低和功能活动早期改变。然而，I 期和 II 期外源代谢在解冻后仍然活跃，至少在短时间内如此。此外，当这些细胞具有高能量状态、在凝胶中固定后进行冷冻保存、在Percoll梯度上与死细胞分离或置于更有利的培养条件下（例如与肝脏非实质细胞共培养）时，可以改善冷冻肝细胞的存活和功能。需要进行更多研究来改进冻融方案并更好地表征储存后的肝实质细胞，包括评估其对特定诱导剂的反应性。 (C) 1999 Elsevier Science Ireland Ltd. 保留所有权利。
Cryopreservation in liquid nitrogen is presently the only way for long-term storage of isolated hepatocytes. Freeze-thaw conditions are not well defined yet; the most critical parameters appear to be the choice of the cryoprotectant, composition of the freezing medium, and cooling and thawing rates. Comparable results have been obtained with hepatocytes from Various species, including man. Cryopreservation usually results in low cell recovery and early alterations of functional activities. However, both phase I and phase II xenobiotic metabolism is still active after thawing, at least during a short period. Moreover, survival and function of cryopreserved hepatocytes can be improved when these cells have a high energy status, are cryopreserved after immobilization in a gel, separated from dead cells on a Percoll gradient or placed in more favorable culture conditions (e.g. in coculture with liver non parenchymal cells). Additional studies are needed to improve freeze-thaw protocols and to better characterize liver parenchymal cells after storage, including evaluation of their responsiveness to specific inducers. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.