Immunohistochemical analysis of the colocalization of corticotropin-releasing hormone receptor and glucocorticoid receptor in kisspeptin neurons in the hypothalamus of female rats
Immunohistochemical analysis of the colocalization of corticotropin-releasing hormone receptor and glucocorticoid receptor in kisspeptin neurons in the hypothalamus of female rats
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DOI：
10.1016/j.neulet.2012.10.010
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发表时间：
2012-11-30
影响因子：
2.5
通讯作者：
Ozawa, Hitoshi
中科院分区：
文献类型：
--
作者：
Takumi, Ken;Iijima, Norio;Ozawa, Hitoshi
Kisspeptin是由Kiss 1基因编码的一种神经肽，在生殖功能的调节中起着重要作用。最近，各种应激源和应激诱导的分子，如促肾上腺皮质激素释放激素（CRH）和皮质酮已被证明抑制吻？在大鼠下丘脑的表达。为了确定CRH和糖皮质激素是否直接作用于kisspeptin神经元，我们研究了CRH受体（CRH-R）和糖皮质激素受体（GR）在雌性大鼠下丘脑kisspeptin神经元的共定位。免疫组化双标记显示，在前腹侧室周核、室周核连续体（AVPV/Pen）和弓状核（ARC）中，多数kisspeptin神经元表达CRH-R。在AVPV/Pen和ARC的部分kisspeptin神经元上也可见CRH免疫反应阳性纤维的密集排列。另一方面，大多数kisspeptin神经元在AVPV/Pen表达GR，而只有少数的kisspeptin神经元在ARC中表达GR。总之，我们的研究提供了神经解剖学证据的kisspeptin神经元的直接调制的CRH和糖皮质激素，并表明，应激诱导的CRH和糖皮质激素抑制促性腺激素分泌通过kisspeptin系统。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Kisspeptin, a neuropeptide encoded by Kiss1 gene, plays pivotal roles in the regulation of reproductive function. Recently various stressors and stress-induced molecules such as corticotropin-releasing hormone (CRH) and corticosterone have been shown to inhibit Kiss? expression in rat hypothalamus. To determine whether CRH and glucocorticoids directly act on kisspeptin neurons, we examined the colocalization of CRH receptor (CRH-R) and glucocorticoid receptor (GR) in kisspeptin neurons in the female rat hypothalamus. Double-labeling immunohistochemistry revealed that most kisspeptin neurons in the anteroventral periventricular nucleus and periventricular nucleus continuum (AVPV/PeN), and arcuate nucleus (ARC) expressed CRH-R. We also observed a few close appositions of CRH immunoreactive fibers on some of kisspeptin neurons in AVPV/PeN and ARC. On the other hand, most kisspeptin neurons in AVPV/PeN expressed GR, whereas only a few of kisspeptin neurons in ARC expressed GR.Altogether, our study provides neuroanatomical evidence of the direct modulation of kisspeptin neurons by CRH and glucocorticoids and suggests that stress-induced CRH and glucocorticoids inhibit gonadotropin secretion via the kisspeptin system. (C) 2012 Elsevier Ireland Ltd. All rights reserved.