A caspase-related protease regulates apoptosis in yeast
A caspase-related protease regulates apoptosis in yeast
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DOI：
10.1016/s1097-2765(02)00501-4
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发表时间：
2002-04-01
期刊：
影响因子：
16
通讯作者：
Fröhlich, KU
中科院分区：
文献类型：
--
作者：
Madeo, F;Herker, E;Fröhlich, KU
酵母可以经历伴随细胞凋亡标记物的细胞死亡。然而，酵母基因组中似乎缺失了经典哺乳动物细胞凋亡调节因子的直向同源物，这对酵母和哺乳动物细胞凋亡的共同机制提出了挑战。在这里，我们研究了 Yor197w，一种与哺乳动物半胱天冬酶结构同源的酵母蛋白，并证明了该蛋白的半胱天冬酶样加工。过氧化氢处理可诱导酵母细胞凋亡以及半胱天冬酶样酶活性。这种反应在破坏后完全消除，并在 Yor197w 过度表达后强烈刺激。 Yor197w 还在按时间顺序老化的培养物中介导死亡过程，这表明它在消除过度老化的细胞中具有生理作用。我们得出结论，Yor197w 确实在酵母中充当真正的 caspase，并提出名称 Yeast Caspase-1（YCA1，基因 YCA1）。
Yeast can undergo cell death accompanied by cellular markers of apoptosis. However, orthologs of classical mammalian apoptosis regulators appeared to be missing from the yeast genome, challenging a common mechanism of yeast and mammalian apoptosis. Here we investigate Yor197w, a yeast protein with structural homology to mammalian caspases, and demonstrate caspase-like processing of the protein. Hydrogen peroxide treatment induces apoptosis together with a caspase-like enzymatic activity in yeast. This response is completely abrogated after disruption and strongly stimulated after overexpression of Yor197w. Yor197w also mediates the death process within chronologically aged cultures, pointing to a physiological role in elimination of overaged cells. We conclude that Yor197w indeed functions as a bona fide caspase in yeast and propose the name Yeast Caspase-1 (YCA1, gene YCA1).