THE CELLULAR 107K-PROTEIN THAT BINDS TO ADENOVIRUS-E1A ALSO ASSOCIATES WITH THE LARGE T-ANTIGENS OF SV40 AND JC VIRUS
THE CELLULAR 107K-PROTEIN THAT BINDS TO ADENOVIRUS-E1A ALSO ASSOCIATES WITH THE LARGE T-ANTIGENS OF SV40 AND JC VIRUS
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DOI：
10.1016/0092-8674(89)90839-8
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发表时间：
1989-07-28
期刊：
影响因子：
64.5
通讯作者：
HARLOW, E
中科院分区：
文献类型：
--
作者：
DYSON, N;BUCHKOVICH, K;HARLOW, E
视网膜母细胞瘤蛋白（p105-RB）与SV 40的大T抗原或腺病毒的E1 A蛋白之间的关联被认为是这些病毒癌基因转化的重要步骤。E1 A和大T抗原共享一个小的氨基酸同源区域，这是与p105-RB高亲和力结合所必需的。该同源区域的突变显示出显著降低由E1 A或大T癌基因介导的转化的频率。以前，E1 A中的这个小区域被证明足以与107，000道尔顿（107 K）的第二种细胞蛋白相互作用。在这里，我们表明，在人类细胞中，SV 40或JC病毒的大T抗原也与107 K形成复合物。107 K与SV 40和JC病毒的大T抗原之间的复合物的证明表明，这些关联可能代表腺病毒和多瘤病毒之间转化的共同机制的另一个组成部分。
The association between the retinoblastoma protein (p105-RB) and either the large T antigen of SV40 or the E1A proteins of adenovirus is thought to be an important step in transformation by these viral oncogenes. E1A and large T antigen share a small region of amino acid homology that is necessary for high affinity binding with p105-RB. Mutations of this homology region were shown to reduce drastically the frequency of transformation mediated by the E1A or large T oncogenes. Previously, this small region in E1A was shown to be sufficient for interaction with a second cellular protein of 107,000 daltons (107K). Here we show that in human cells, the large T antigens of SV40 or JC virus also from complexes with 107K. Demonstration of complexes between 107K and the large T antigens of SV40 and JC virus suggests that these associations may represent another component of a common mechanism for transformation between adenoviruses and polyoma viruses.