Enhanced anaerobic dechlorination of polychlorinated biphenyl in sediments by bioanode stimulation.
Enhanced anaerobic dechlorination of polychlorinated biphenyl in sediments by bioanode stimulation.
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DOI：
10.1016/j.envpol.2015.12.039
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发表时间：
2016-04
影响因子：
8.9
通讯作者：
Hui Yu;C. Feng;Xiaoping Liu;X. Yi;Yuan Ren;Chao-hai Wei
中科院分区：
文献类型：
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作者：
Hui Yu;C. Feng;Xiaoping Liu;X. Yi;Yuan Ren;Chao-hai Wei
应用低压电场作为电子供体或受体来促进氯化有机化合物的生物修复是一项很有前途的技术，可以满足开发高效和经济的策略来原位处理多氯联苯污染沉积物的需求。在这里，我们报道了具有阳极电位的生物阳极刺激显著增强了中国广东清远电子垃圾回收场沉积物中含有的2,3,4,5-四氯联苯（PCB 61）的脱氯。在相对于饱和甘汞电极的0.2 V电位条件下，生物阳极孵育110天，在初始浓度为100 μmol kg−1时，总PCB 61的转化率为58%，而在开路参考实验中仅降低23%。在生物电化学反应器（BER）中引入醋酸盐进一步将PCB 61的转化率提高到82%。对细菌组成的分析表明，随着处理的变化，群落发生了显著的变化。在门水平上，生物阳极刺激导致factinobacteria、Bacteroidetes和chloroflexia丰度大幅增加，这些细菌要么能够脱氯，要么在PCB污染的环境中检测到。在属水平上，BER包含两种类型的微生物：以geobacter、Ignavibacterium和dysgonomonas为代表的电化学活性细菌（EAB）和以hydrogenophaga、Alcanivorax、Sedimentibacter、Dehalogenimonas、ComamonasandVibrio为代表的脱氯细菌。这些结果表明，EAB的存在可以促进负责PCB 61转化的脱氯细菌的数量。
The application of a low-voltage electric field as an electron donor or acceptor to promote the bioremediation of chlorinated organic compounds represents a promising technology meeting the demand of developing an efficient and cost-effective strategy forin situtreatment of PCB-contaminated sediments. Here, we reported that bioanode stimulation with an anodic potential markedly enhanced dechlorination of 2,3,4,5-tetrachlorobiphenyl (PCB 61) contained in the sediment at an electronic waste recycling site of Qingyuan, Guangdong, China. The 110-day incubation of the bioanode with a potential poised at 0.2 V relative to saturated calomel electrode enabled 58% transformation of the total PCB 61 at the initial concentration of 100 μmol kg−1, while only 23% was reduced in the open-circuit reference experiment. The introduction of acetate to the bioelectrochemical reactor (BER) further improved PCB 61 transformation to 82%. Analysis of the bacterial composition showed significant community shifts in response to variations in treatment. At phylum level, the bioanode stimulation resulted in substantially increased abundance ofActinobacteria,Bacteroidetes, andChloroflexieither capable of PCB dechlorination, or detected in the PCB-contaminated environment. At genus level, the BER contained two types of microorganisms: electrochemically active bacteria (EAB) represented byGeobacter,Ignavibacterium, andDysgonomonas, and dechlorinating bacteria includingHydrogenophaga,Alcanivorax,Sedimentibacter,Dehalogenimonas,ComamonasandVibrio. These results suggest that the presence of EAB can promote the population of dechlorinating bacteria which are responsible for PCB 61 transformation.