Adhesion of the positively charged bacterium Stenotrophomonas (Xanthomonas) maltophilia 70401 to glass and teflon
Adhesion of the positively charged bacterium Stenotrophomonas (Xanthomonas) maltophilia 70401 to glass and teflon
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DOI：
10.1128/jb.178.18.5472-5479.1996
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发表时间：
1996-09-01
影响因子：
3.2
通讯作者：
Zehnder, AJB
中科院分区：
文献类型：
--
作者：
Jucker, BA;Harms, H;Zehnder, AJB
医疗植入物经常被细菌定植，这可能导致严重感染。定植的初始步骤，即细菌粘附到人工固体表面，主要由固体表面和细菌细胞之间的长程货车德瓦尔斯和静电相互作用控制。虽然货车范德华力通常是吸引力，但细菌和固体表面通常带负电荷，导致静电排斥。我们在这里报告的粘附的临床分离株，嗜麦芽窄食单胞菌70401，这是在生理pH值，带正电荷。S.嗜麦芽窄食单胞菌的电泳迁移率为+0.3。10（-8）m（2）V（-1）s（-1），pH 11。正电荷可能来源于位于外膜的蛋白质。对于这种细菌来说，参与粘附的两种长程力都是有吸引力的。因此，S.与带负电荷的细菌恶臭假单胞菌MT 2相比，嗜麦芽窄食单胞菌对带负电荷的表面如玻璃和特氟隆更有利。在低离子强度的介质中，由于带负电荷的扩散层较厚，带负电荷的细菌的粘附受到阻碍。嗜麦芽窄食单胞菌在稀培养基中特别有利。S.在不同离子强度下的嗜麦芽窄食单胞菌可以用计算的S.嗜麦芽窄食菌和玻璃或聚四氟乙烯。
Medical implants are often colonized by bacteria which may cause severe infections. The initial step in the colonization, the adhesion of bacteria to the artificial solid surface, is governed mainly by long-range van der Waals and electrostatic interactions between the solid surface and the bacterial cell. While van der Waals forces are generally attractive, the usually negative charge of bacteria and solid surfaces leads to electrostatic repulsion. We report here on the adhesion of a clinical isolate, Stenotrophomonas maltophilia 70401, which is, at physiological pH, positively charged. S. maltophilia has an electrophoretic mobility of +0.3 . 10(-8)m(2)V(-1)s(-1) at pH 7 and an overall surface isoelectric point at pH 11. The positive charge probably originates from proteins located in the outer membrane. For this bacterium, both long-range forces involved in adhesion are attractive. Consequently, adhesion of S. maltophilia to negatively charged surfaces such as glass and Teflon is much favored compared with the negatively charged bacterium Pseudomonas putida mt2. While adhesion of negatively charged bacteria is impeded in media of low ionic strength because of a thick negatively charged diffuse layer, adhesion of S. maltophilia was particularly favored in dilute medium. The adhesion efficiencies of S. maltophilia at various ionic strengths could be explained in terms of calculated long-range interaction energies between S. maltophilia and glass or Teflon.