The morphogenesis of basal bodies and accessory structures of the cortex of the ciliated protozoan Tetrahymena pyriformis.
The morphogenesis of basal bodies and accessory structures of the cortex of the ciliated protozoan Tetrahymena pyriformis.
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DOI：
10.1083/jcb.40.3.716
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发表时间：
1969-03
期刊：
影响因子：
--
通讯作者：
Allen RD
中科院分区：
文献类型：
--
作者：
Allen RD
通过透射电子显微镜观察梨形四膜虫的分裂细胞的皮质结构形态发生的迹象。观察到的基体发育的最早阶段是由九个单管组成的短圆柱体，通过内部车轮结构连接。它在横向微管下方且靠近基底微管的前近端表面处垂直于成熟基底体。连续阶段表明，单管变成三联管，然后“基体”伸长并向生物体表面倾斜，同时与“亲代”保持 75-100 mμ 的恒定距离。新基体在完全伸展后接触表膜，然后呈现与母体基体平行的方向并移动到母体基体的前面。在发育中的基体伸长后，基体管腔中的不电子透明核心和其外近端表面周围的附属结构出现。这些附属结构与其他基体的对应结构以及纵向微管相结合，可能在基体的最终定位中发挥作用，从而在皮质模式的维持中发挥作用。对基体形成的第二序列的观察表明，口腔原基是由体细胞基体的多次复制产生的。
Dividing cells of Tetrahymena pyriformis were observed by transmission electron microscopy for signs of morphogenesis of cortical structures. The earliest stage of basal body development observed was of a short cylinder of nine single tubules connected by an internal cartwheel structure. This is set perpendicular to the mature basal body at its anterior proximal surface under the transverse microtubules and next to the basal microtubules. Sequential stages show that the single tubules become triplet tubules and that the "probasal bodies" then elongate and tilt toward the organism's surface while maintaining a constant distance of 75–100 mµ with the "parent." The new basal body after it is fully extended contacts the pellicle, and then assumes a parallel orientation with and moves anterior to the parent basal body. The electron-opaque core in the lumen of the basal body and accessory structures around its outer proximal surface appear after the developing basal body has elongated. These accessory structures associating with their counterparts from other basal bodies and with the longitudinal microtubules may play a role in the final positioning of basal bodies and thus in the maintenance of cortical patterns. Observations on a second sequence of basal body formation suggest that the oral anlage arises by multiple duplication of somatic basal bodies.