Particle separation using ultrasound can radically reduce embolic load to brain after cardiac surgery
Particle separation using ultrasound can radically reduce embolic load to brain after cardiac surgery
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DOI：
10.1016/j.athoracsur.2004.04.071
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发表时间：
2004-11-01
影响因子：
4.6
通讯作者：
Laurell, T
中科院分区：
文献类型：
--
作者：
Jönsson, H;Holm, C;Laurell, T
背景。体外循环期间的微栓塞被认为是心脏手术后神经认知障碍的主要原因。在体外循环过程中，通常再输入患者体内的血液是脑毛细血管中脂质微栓子的主要来源。介绍了一种基于声驻波分离微通道中流体颗粒的新技术，该技术具有去除血液中脂质颗粒的能力。由8个平行的高保真微加工通道组成的分离器在超声场的驱动下产生驻波。随着血细胞比容的增加，在血液中加入三种不同浓度的脂质颗粒（直径均为0.3 μ m），并将其引入分离通道，使脂质颗粒与红细胞分离。脂质颗粒的平均分离率为81.9% +/- 7.6%，红细胞的平均分离率为79.8% +/- 9.9%，两者均与入血样本的红细胞压积水平有关。该手术是非创伤性的，没有引起溶血。利用声驻波技术进行颗粒分离可用于无创有效地去除血液中的脂质颗粒，在减少体外循环后神经认知并发症方面可能具有临床意义。(C) 2004年由胸外科学会出版。
Background. Microembolism during cardiopulmonary bypass has been suggested as being the predominant cause of neurocognitive disorders after cardiac surgery. Shed blood, normally retransfused into the patient during cardiopulmonary bypass, is a major source of lipid microemboli in the brain capillaries. A newly developed technique based on acoustic standing-wave separation of particles in fluid in microchannels, with the capacity to remove lipid particles in blood, is presented.Methods. A separator consisting of eight parallel, high-fidelity microfabricated channels was actuated with an ultrasound field to create a standing wave. Three different concentrations of lipid particles (diameter, 0.3 mum) were added to blood samples with increasing hematocrits and introduced into the separator channels to separate lipid particles and erythrocytes.Results. The mean separation rates for lipid particles were 81.9% +/- 7.6% and for erythrocytes 79.8% +/- 9.9%, and both were related to the hematocrit level of the incoming blood sample. The procedure was atraumatic and did not cause hemolysis.Conclusions. Particle separation by means of an acoustic standing-wave technique can be used for atraumatic and effective removal of lipid particles from blood, with the possible clinical implication of reducing neurocognitive complications after cardiopulmonary bypass. (C) 2004 by The Society of Thoracic Surgeons.