Single-cell analysis for BDNF and TrkB receptors in cardiac microvascular endothelial cells
Single-cell analysis for BDNF and TrkB receptors in cardiac microvascular endothelial cells
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心脏微血管内皮细胞 BDNF 和 TrkB 受体的单细胞分析
DOI：
10.3233/bme-141038
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发表时间：
2014-01-01
影响因子：
1
通讯作者：
Cai, Dongqing
中科院分区：
文献类型：
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作者：
Bai, Xinlei;Chen Yilin;Cai, Dongqing
近年来研究发现，BDNF-TrkB通路在心肌微血管内皮细胞（CMECs）介导的心肌血管新生中发挥重要作用。单细胞分析是研究个体基因表达的有力工具，因为单个细胞将经历细胞异质性和动态微环境。目前对BDNF和TrkB受体在单个CMEC水平的表达知之甚少。我们对7个随机选择的CMECs的BDNF和TrkB受体（FL，T1，T2）的单细胞分析表明，在体外培养环境下，BDNF在7个选择的CMECs中的2个中表达。所有7个CMEC均不表达TrkB-FL，TrkB-T1在所有7个CMEC中表达，而TrkB-T2在其中3个中表达。此外，没有一个CMEC同时表达BDNF和三种TrkB受体或BDNF和TrkB-FL。这些结果提示，BDNF及其受体在CMEC中的表达可能是随机的或随机的，以响应时间和空间变化、调节或病理生理变化的需要。
Recent studies revealed that BDNF-TrkB pathway plays an important role in cardiac microvascular endothelial cells (CMECs) mediated myocardial angiogenesis. Single-cell analysis is a powerful tool for studying gene expression in individuals since cellular heterogeneity and dynamic microenvironments which individual cell will experience. Little is currently known about the expression of BDNF and TrkB receptors at the single CMEC level. Our single-cell analysis of seven randomly selected CMECs for BDNF and TrkB receptors (FL, T1, T2) showed that under an in vitro culture environment, BDNF was expressed in two of the seven selected CMECs. None of the single CMEC expressed TrkB-FL. TrkB-T1 was expressed in all seven selected CMECs, while, TrkB-T2 was expressed in three of these. In addition, none of single CMEC was found to express both BDNF and three TrkB receptors or BDNF and TrkB-FL simultaneously. These results suggest that a stochastic or random expression pattern for BDNF and their receptors might be set in each of the CMEC to response requirement of the time and spatial change, regulation or pathophysiological change.