Graphene quantum dots-based platform for the fabrication of electrochemical biosensors
Graphene quantum dots-based platform for the fabrication of electrochemical biosensors
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DOI：
10.1016/j.elecom.2010.11.005
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发表时间：
2011-01-01
影响因子：
5.4
通讯作者：
Li, Genxi
中科院分区：
文献类型：
--
作者：
Zhao, Jing;Chen, Guifang;Li, Genxi
基于单链DNA（ssDNA）与石墨烯材料之间的强相互作用，我们设计了一种简单而智能的平台，利用石墨烯量子点修饰的热解石墨电极偶联特定序列的ssDNA分子作为探针，构建了电化学生物传感器。由于石墨烯材料优异的导电性，修饰电极可以表现出非常精细的电化学响应。然而，探针ssDNA将抑制电化学活性物种[Fe（CN）（6）]（3-/4-）和电极之间的电子转移后，探针分子通过它们与石墨烯的相互作用而强烈地结合到修饰电极的表面。然而，当测试溶液中还存在靶分子如靶ssDNA或靶蛋白质时，如果探针ssDNA的序列被设计为与靶DNA互补或作为靶蛋白质的适体，则探针ssDNA将与靶结合而不是与石墨烯结合。结果表明，[Fe（CN）（6）]（3-/4-）的峰电流随着目标分子的增加而增加，从而可以方便地开发各种电化学生物传感器。所制备的电化学生物传感器也可能具有高的灵敏度和选择性，这可能具有潜在的应用前景。(C)2010 Elsevier B. V.保留所有权利。
Based on the strong interaction between single-stranded DNA (ssDNA) and graphene material, we have designed a simple but smart platform in this work to fabricate electrochemical biosensors by using graphene quantum dots modified pyrolytic graphite electrode coupled with specific sequence ssDNA molecules as probes. Due to the excellent conductivity of graphene material, the modified electrode can exhibit very fine electrochemical response. Nevertheless, the probe ssDNA will inhibit the electron transfer between the electrochemical active species [Fe(CN)(6)](3-/4-) and the electrode after the probe molecules are strongly bound to the surface of the modified electrode via their interaction with graphene. However, when the target molecules such as target ssDNA or target protein also exist in the test solution, the probe ssDNA will bind with the target instead of graphene if the sequence of the probe ssDNA is designed as complementary to the target DNA or as the aptamer of the target protein. As a result, the obtained peak currents of [Fe(CN)(6)](3-/4-) will increase with the target molecules, thus various electrochemical biosensors can be easily developed with this proposed platform. The fabricated electrochemical biosensors may also have high sensitivity and selectivity, which may have potential applications in the future. (C) 2010 Elsevier B.V. All rights reserved.