Clinicopathologic Application of Lectin Histochemistry Bisecting GlcNAc in Glioblastoma
Clinicopathologic Application of Lectin Histochemistry Bisecting GlcNAc in Glioblastoma
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DOI：
10.1097/pai.0b013e3181e3bf0d
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发表时间：
2010-12-01
影响因子：
1.6
通讯作者：
Tanaka, Shinya
中科院分区：
文献类型：
--
作者：
Aoyanagi, Eiko;Sasai, Ken;Tanaka, Shinya
糖基化是最常见的翻译后修饰之一，寡糖结构的变化与许多人类疾病（包括许多癌症）相关。因此，发现作为脑肿瘤进展和转移标志物的异常糖基化模式是改善临床病理诊断并为新疗法的开发提供帮助的一种有吸引力的策略。为了鉴定表达含有高水平平分 N-乙酰氨基葡萄糖 (GlcNAc) 结构的糖蛋白的胶质母细胞瘤 (GBM) 细胞，使用红细胞凝集植物血凝素进行了凝集素组织化学分析。尽管 GBM 经常表达平分 GlcNAc，但各个样本内的肿瘤区域之间的凝集素反应性有所不同。由于详细的组织病理学分析表明，具有平分 GlcNAc 结构的寡糖优选在 KI67 免疫阳性率较低的肿瘤区域中表达，因此平分 GlcNAc 的免疫检测可用于指示人 GBM 中增殖较少的区域。我们的研究强调了凝集素组织化学开发新诊断方法的潜在用途，从而改善未来的抗神经胶质瘤治疗。
Glycosylation is one of the most common posttranslational modifications and changes in oligosaccharide structures are associated with many human diseases including a number of cancers. Thus, discovering aberrant glycosylation patterns that serve as markers for brain tumor progression and metastasis represents an attractive strategy to improve clinicopathologic diagnosis and to provide aids to the development of novel therapies. To identify glioblastoma (GBM) cells expressing glycoproteins that contain high levels of the bisecting N-acetylglucosamine (GlcNAc) structures, lectin histochemistry was carried out using erythroagglutinating phytohemagglutinin. Although GBM frequently expressed the bisecting GlcNAc, the lectin reactivity varied among tumor regions within individual specimens. Since detailed histopathologic analysis revealed that oligosaccharides with bisecting GlcNAc structures were preferably expressed in tumor regions with low KI67 immunopositivity, immunodetection of the bisecting GlcNAc could be useful to indicate less proliferative regions in human GBM. Our study highlights the potential use of lectin histochemistry to develop new methods for diagnosis that would improve future antiglioma therapy.