EARLY ULTRASTRUCTURAL EFFECTS OF GENTAMICIN COCHLEOTOXICITY
EARLY ULTRASTRUCTURAL EFFECTS OF GENTAMICIN COCHLEOTOXICITY
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DOI：
10.3109/00016489109137387
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发表时间：
1991-01-01
影响因子：
1.4
通讯作者：
VELDMAN, JE
中科院分区：
文献类型：
--
作者：
DEGROOT, JCMJ;HUIZING, EH;VELDMAN, JE
本文观察了庆大霉素中毒早期耳蜗毛细胞经三醛固定和OsO_4/K_4Ru（CN）_6固定后的超微结构变化。 在用庆大霉素处理5、10和15天的耳蜗中，单个外毛细胞（OHC 1、OHC 2和OHC 3）被随机影响。 在此阶段未观察到内毛细胞变性。 在超微结构上，最早的可识别的变化是溶酶体的增加，内质网的增殖和亨森小体的形成。 随后内质网和核膜扩张，引起广泛的细胞质空泡化。 这些结果表明庆大霉素诱导的变化主要涉及细胞的合成装置。 所有进一步的细胞内变化都发生在更晚的阶段，因此被认为是继发性事件。
Ultrastructural changes in the cochlear hair cells during the early stages of gentamicin intoxication were investigated after tri-aldehyde primary fixation and OsO4/K4Ru(CN)6 post-fixation. In cochleas treated for 5, 10 and 15 days with gentamicin the individual outer hair cells (OHC1, OHC2 and OHC3) were randomly affected. Degeneration of the inner hair cells was not observed at this stage. Ultrastructurally, the earliest identifiable changes were an increase in lysosomes, proliferation of the endoplasmic reticulum, and formation of Hensen's bodies. This was followed by dilatation of the endoplasmic reticulum and the nuclear envelope, giving rise to extensive cytoplasmic vacuolation. These results demonstrate that gentamicin-induced changes primarily involve the cell's synthetic apparatus. All further intracellular changes occur at a much later stage and are therefore to be considered secondary events.