A comparison of synteny and gene order on the homologue of chicken chromosome 7 between two passerine species and between passerines and chicken.
A comparison of synteny and gene order on the homologue of chicken chromosome 7 between two passerine species and between passerines and chicken.
复制标题
两种雀形目鸟类以及雀形目动物和鸡之间鸡7号染色体同源物的同线性和基因顺序比较。
DOI：
10.1159/000125837
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发表时间：
2008
影响因子：
1.7
通讯作者：
Hale MC
中科院分区：
文献类型：
--
作者：
Hale MC
雀形目鸟类的野生种群经常被用于生态学和进化的研究。然而，大多数物种缺乏遗传连锁图谱。完成一个模型鸟类基因组序列（原鸡，原鸡）创造了一个机会，以推进其他鸟类的遗传知识。在这里，我们构建了两个雀形目物种，斑胸草雀（Taeniopygia guttata）和家雀（Passer arteriticus）的鸡7号染色体同源基因的遗传连锁图谱。连锁群的构建与SNP和微卫星标记的组合，这是在硅片上开发的基础上的鸡基因组序列的相似性，并在实验室从头开始。同线性在整个染色体上是很保守的，但是在鸡和雀形目之间存在一些基因重排。这表明，转移的地图信息从鸡雀形目和不同雀形目物种之间不应该假设保守的基因顺序。7号染色体连锁图谱的长度平均为鸡的60%，这与雀形目鸟类相对于鸡重组率降低的观点一致。观察到了异交的一些证据，即性别之间重组率的差异。
Wild populations of passerine birds have frequently been used in studies of ecology and evolution. However, the majority of species lack genetic linkage maps. The completion of a model avian genome sequence (the jungle fowl,Gallus gallus) has created an opportunity to advance genetic knowledge of other birds. Here we constructed genetic linkage maps of the homologue of chicken chromosome 7 for two passerine species, the zebra finch (Taeniopygia guttata) and the house sparrow (Passer domesticus). Linkage groups were constructed with a combination of SNP and microsatellite markers that were developed both in silico based on similarity to the chicken genome sequence, and de novo in the laboratory. Synteny was well conserved throughout the chromosome; however there were some gene rearrangements between chickens and passerines. This suggests that the transfer of map information from chicken to passerine and between different passerine species should not assume conserved gene order. The length of linkage maps of chromosome 7 were on average 60% that of chicken, consistent with the idea that passerines have a reduced recombination rate relative to chicken. Some evidence of heterochiasmy, i.e. a difference in the recombination rate between the sexes, was observed.