Polyoma virus mutants as probes of variety among mouse embryonal carcinoma cell lines.
Polyoma virus mutants as probes of variety among mouse embryonal carcinoma cell lines.
复制标题
多瘤病毒突变体作为小鼠胚胎癌细胞系中多样性的探针。
DOI：
10.1111/j.1432-0436.1982.tb01225.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
D. Blangy
中科院分区：
文献类型：
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作者：
E. Georges;M. Vasseur;D. Blangy
胚胎癌细胞（EC）对多瘤病毒的感染有抵抗力，并且在允许分化时变得宽容。在两种胚胎癌细胞系 PCC4 和 F9 上选择了多瘤宿主范围 (PyEC) 突变体，这两种细胞系在体外和体内的分化潜力均不同。由于在一个品系上选择的 PyEC 突变体未能发育，或在另一个品系上仅发育不良，因此我们使用这两类突变体作为探针，针对几个 EC 品系，这些品系的起源和分化特性不同。根据它们对任一突变体的敏感性，以及温度对感染效率的影响，我们推断出这些畸胎癌细胞系的分类，这与之前基于代谢耦合的分类一致。我们还讨论了表明不同 EC 细胞系可能在胚胎水平的细胞测定过程中呈现步骤的结果。
Embryonal carcinoma (EC) cells are resistant to infection with polyoma virus and become permissive when allowed to differentiate. Polyoma host-range (PyEC) mutants have been selected on two embryonal carcinoma cell lines, PCC4 and F9, which differ in their differentiation potential, both in vitro and in vivo. Since PyEC mutants selected on one line failed to develop, or developed only poorly on the other, we used these two classes of mutants as probes towards several EC lines which differed in their origin and differentiation properties. From their susceptibility to either mutant, and from the effect of temperature upon the efficiency of infection, we inferred a classification of these teratocarcinoma cell lines, which is an agreement with a previous one based upon metabolic coupling. We also discussed results indicating that different EC cell lines might present steps in the process of cell determination at the embryonic level.