Prediction of chromatographic retention and protein identification in liquid chromatography/mass spectrometry
Prediction of chromatographic retention and protein identification in liquid chromatography/mass spectrometry
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DOI：
10.1021/ac0256890
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发表时间：
2002-11-15
影响因子：
7.4
通讯作者：
Bergquist, J
中科院分区：
文献类型：
--
作者：
Palmblad, M;Ramström, M;Bergquist, J
液相色谱与质谱联用的在线或离线技术作为蛋白质组学的一种工具正在迅速发展，它能够处理生物学中固有的复杂性，并补充基于二维凝胶电泳的传统方法。蛋白质可以通过蛋白水解消化和肽质量指纹图谱或通过使用串联质谱法产生的短序列标签搜索数据库来鉴定。本文表明，肽的色谱行为的信息，可以协助蛋白质鉴定的肽质量指纹在液相色谱/质谱。这些额外的信息是重要的，并且已经可以在没有额外的实验成本。
Liquid chromatography coupled on- or off-line with mass spectrometry is rapidly advancing as a tool in proteomics capable of dealing with the inherent complexity in biology and complementing conventional approaches based on two-dimensional gel electrophoresis. Proteins can be identified by proteolytic digestion and peptide mass fingerprinting or by searching databases using short-sequence tags generated by tandem mass spectrometry. This paper shows that information on the chromatographic behavior of peptides can assist protein identification by peptide mass fingerprinting in liquid chromatography/mass spectrometry. This additional information is significant and already available at no extra experimental cost.