Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.
Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques.
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DOI：
10.1371/journal.pone.0171148
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Van Rompay KK
中科院分区：
文献类型：
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作者：
Coffey LL;Pesavento PA;Keesler RI;Singapuri A;Watanabe J;Watanabe R;Yee J;Bliss-Moreau E;Cruzen C;Christe KL;Reader JR;von Morgenland W;Gibbons AM;Allen AM;Linnen J;Gao K;Delwart E;Simmons G;Stone M;Lanteri M;Bakkour S;Busch M;Morrison J;Van Rompay KK
需要寨卡病毒(ZIKV)的动物模型来更好地了解寨卡病毒的趋向性和致病机理，并测试候选疫苗和治疗方法以遏制疫情。人类和恒河猴拥有相似的胎儿发育和胎盘生物学，这是人类和啮齿动物所不具备的。我们用2015年的巴西ZIKV毒株接种了两只未怀孕的恒河猴。与大多数人类感染一样，这些动物没有经历临床疾病，但出现了短暂的血浆病毒病，随着中和抗体的产生而清除。在1只动物中，病毒RNA(VRNA)在全血中的检测时间比在血浆中的时间更长。尽管没有重大的组织病理学变化，但许多成人组织在感染后14天含有vRNA，其中血淋巴组织中的vRNA水平最高。这些观察结果值得进一步研究，以调查ZIKV的持久性及其通过血液产品或组织和器官移植传播的潜在临床意义。
Animal models of Zika virus (ZIKV) are needed to better understand tropism and pathogenesis and to test candidate vaccines and therapies to curtail the pandemic. Humans and rhesus macaques possess similar fetal development and placental biology that is not shared between humans and rodents. We inoculated 2 non-pregnant rhesus macaques with a 2015 Brazilian ZIKV strain. Consistent with most human infections, the animals experienced no clinical disease but developed short-lived plasma viremias that cleared as neutralizing antibody developed. In 1 animal, viral RNA (vRNA) could be detected longer in whole blood than in plasma. Despite no major histopathologic changes, many adult tissues contained vRNA 14 days post-infection with highest levels in hemolymphatic tissues. These observations warrant further studies to investigate ZIKV persistence and its potential clinical implications for transmission via blood products or tissue and organ transplants.