Measuring Microfibrillar Angles Using Light Microscopy
Measuring Microfibrillar Angles Using Light Microscopy
复制标题
使用光学显微镜测量微纤维角度
作者：
J. Senft;B. Bendtsen
本文回顾了一种快速、可靠的增强木材细胞壁微纤维角度的方法，该方法是对I.W.Bailey技术的改进。干燥的切片用碘化钾溶液和硝酸处理，在针叶木管胞或阔叶木纤维的细胞壁中产生晶体。晶体与主要的微纤维角度对准，可以很容易地观察和测量。
A rapid, reliable method of enhancing microfibrillar angles for viewing in wood cell walls, a modification of I. W. Bailey's technique, is reviewed. Dried microtomed sections are treated with an iodinepotassium iodide solution and nitric acid to produce crystals in cell walls of softwood tracheids or hardwood fibers. The crystals are aligned with the predominant microfibrillar angle and may be easily viewed and measured.