Remote control of ion channels and neurons through magnetic-field heating of nanoparticles
Remote control of ion channels and neurons through magnetic-field heating of nanoparticles
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DOI：
10.1038/nnano.2010.125
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发表时间：
2010-08-01
影响因子：
38.3
通讯作者：
Pralle, Arnd
中科院分区：
文献类型：
--
作者：
Huang, Heng;Delikanli, Savas;Pralle, Arnd
最近，光学刺激（1-3）已经开始解开控制某些动物行为的神经元处理（4，5）。然而，光学方法受限于可见光不能深入穿透到组织中。在这里，我们展示了一种基于射频磁场加热纳米颗粒的方法，以远程激活细胞中的温度敏感阳离子通道。超顺磁性铁氧体纳米粒子靶向表达TRPV 1的细胞质膜上的特定蛋白质，并通过射频磁场加热。使用荧光团作为分子温度计，我们表明，诱导的温度升高是高度本地化的。通道的热激活在培养的神经元中触发动作电位，而没有可观察到的毒性作用。这种方法可以适用于刺激其他细胞类型，而且可以用于远程操纵其他细胞机制以用于新的治疗方法。
Recently, optical stimulation(1-3) has begun to unravel the neuronal processing that controls certain animal behaviours(4,5). However, optical approaches are limited by the inability of visible light to penetrate deep into tissues. Here, we show an approach based on radio-frequency magnetic-field heating of nanoparticles to remotely activate temperature-sensitive cation channels in cells. Superparamagnetic ferrite nanoparticles were targeted to specific proteins on the plasma membrane of cells expressing TRPV1, and heated by a radio-frequency magnetic field. Using fluorophores as molecular thermometers, we show that the induced temperature increase is highly localized. Thermal activation of the channels triggers action potentials in cultured neurons without observable toxic effects. This approach can be adapted to stimulate other cell types and, moreover, may be used to remotely manipulate other cellular machinery for novel therapeutics.