Absence of Galectin-1 accelerates CD8+ T cell-mediated graft rejection
Absence of Galectin-1 accelerates CD8+ T cell-mediated graft rejection
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DOI：
10.1002/eji.201142325
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发表时间：
2012-11-01
影响因子：
5.4
通讯作者：
Lombardi, Giovanna
中科院分区：
文献类型：
--
作者：
Moreau, Aurelie;Noble, Alistair;Lombardi, Giovanna
半乳糖凝集素-1（Gal-I）是内源性β-半乳糖结合蛋白家族的成员，具有预防自身免疫性疾病和慢性炎症的作用。在这项研究中，使用Gal-1缺陷小鼠（Gal-1-/-）研究了Gal-1在移植排斥反应中的作用。我们证明，在Gal-1的情况下，与WT小鼠相比，皮肤移植物更早被排斥，这是由于CD 8 + T细胞在移植物排斥反应中所起的作用。Gal-1-/-和WT小鼠之间观察到的移植物存活率的差异可以通过抗原特异性CD 8 + T细胞百分比的增加和Gal-1-/-小鼠中CD 8 + T细胞与WT小鼠相比优先分泌IFN-γ和IL-17来解释。这项研究表明，Gal-1的内源性表达有助于移植物存活。从使用缺乏Gal-1的小鼠获得的结果也证实了CD 8 + T细胞在移植物排斥中的关键作用。
Galectin-1 (Gal-1) is a member of a family of endogenous beta-galactose-binding proteins with a role in preventing autoimmune diseases and chronic inflammation. In this study, the involvement of Gal-1 in graft rejection was investigated by using Gal-1-deficient mice (Gal-1-/-). We demonstrate that in the absence of Gal-1, skin grafts are rejected earlier compared with those of WT mice, and that this is due to the role played by CD8+ T cells in graft rejection. The difference in graft survival observed between Gal-1-/- and WT mice was explained by both an increase in the percentage of antigen-specific CD8+ T cells and by preferential secretion of IFN-gamma and IL-17 by CD8+ T cells in Gal-1-/- mice compared with WT mice. This study suggests that endogenous expression of Gal-1 contributes to graft survival. The results obtained from the use of mice deficient in Gal-1 also confirm a key role for CD8+ T cells in graft rejection.