Immunolocalization of tumor necrosis factor receptor-associated factor 6 in rat periapical lesions.
Immunolocalization of tumor necrosis factor receptor-associated factor 6 in rat periapical lesions.
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DOI：
10.1016/j.joen.2009.03.011
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发表时间：
2009-06
影响因子：
4.2
通讯作者：
Lijie Yang;Chi Zhang;B. Peng
中科院分区：
文献类型：
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作者：
Lijie Yang;Chi Zhang;B. Peng
简介：牙槽骨破坏与破骨细胞活性增强有关;肿瘤坏死因子受体相关因子6（TRAF 6）被认为有助于破骨细胞的分化。本研究旨在观察TRAF 6在大鼠根尖周病变中的免疫组织化学定位及骨吸收情况。方法：采用下颌第一磨牙牙髓暴露法制作根尖周病变模型。在第0、7、14、21和28天随机处死动物。制备额部切片用于免疫组织化学和酶组织化学。结果：培养第7天，可见少量TRAF 6阳性细胞和破骨细胞。两人都在第14天达到高潮。在21天和28天的样本中，TRAF 6表达减少，破骨细胞减少。从第7天到第28天，TRAF 6表达与破骨细胞数量之间存在显著正相关。结论：TRAF 6可能与根尖周病变中破骨细胞的分化有关
Introduction: Alveolar bone destruction is associated with enhanced osteoclast activity; tumor necrosis factor receptor–associated factor 6 (TRAF6) is suggested to contribute to the differentiation of osteoclasts. The purpose of this study was to observe the immunohistochemical localization of TRAF6 and bone resorption in induced periapical lesions in rats. Methods: Periapical lesions were induced by an occlusal pulp exposure in the mandibular first molars. Animals were randomly killed at 0, 7, 14, 21, and 28 days. Frontal sections were prepared for immunohistochemistry and enzyme histochemistry. Results: A few TRAF6-positive cells and osteoclasts could be observed on day 7. Both climaxed on day 14. In the 21-day and 28-day samples, TRAF6 expression decreased, and fewer osteoclasts were observed. From day 7 to day 28, a significant positive correlation was found between TRAF6 expression and osteoclast numbers. Conclusions: These findings showed that TRAF6 might be associated with osteoclast differentiation in periapical lesions