C-type natriuretic peptide/guanylate cyclase B system in ATDC5 cells, a chondrogenic cell line
C-type natriuretic peptide/guanylate cyclase B system in ATDC5 cells, a chondrogenic cell line
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DOI：
10.1007/s007740200019
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发表时间：
2002-01-01
影响因子：
3.3
通讯作者：
Nakao, K
中科院分区：
文献类型：
--
作者：
Suda, M;Tanaka, K;Nakao, K
利钠肽由三种结构相关的肽组成：心房利钠肽（ANP）、脑利钠肽（BNP）和C型利钠肽（CNP）。颗粒鸟苷酸环化酶GC-A和GC-B是这些肽的受体，以介导它们的作用。ANP和BNP对GC-A具有高亲和力，CNP是GC-B的优选配体。在这篇文章中，我们报告了我们的研究的表达和可能的作用（S）利钠肽在ATDC 5细胞，这是一个软骨细胞系。ATDC 5细胞产生环磷酸鸟苷（cGMP）的钠尿肽的反应。就环鸟苷酸的产生而言，CNP比ANP有效得多。通过北方印迹分析证明GC-A和GC-B的信息，并通过Southern印迹结合逆转录-聚合酶链反应（RT-PCR）显示CNP的存在。这些结果表明CNP/GC-B系统优先在ATDC 5细胞中表达。GC-B mRNA表达在融合后14天高于融合时。CNP或8-溴cGMP减少[H-3]胸苷摄取，并略微增加X型胶原的信息。这是肥大软骨细胞的标志。这些数据表明CNP/GC-B系统可能是ATDC 5细胞的自分泌/旁分泌调节剂，从而影响其生长和分化。
Natriuretic peptides constitute a family of three structurally related peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). Particulate guanylate cyclases, GC-A, and GC-B, are the receptors for these peptides to mediate their action. ANP and BNP possess high affinities for GC-A, and CNP is the preferred ligand for GC-B. In this article, we report our study of the expression and possible role(s) of natriuretic peptides in ATDC5 cells, which represent a chondrogenic cell line. ATDC5 cells produced cyclic guanosine monophosphate (cGMP) in response to natriuretic peptides. CNP was far more potent than ANP in terms of cGMP production. The messages for GC-A and GC-B were demonstrated by means of Northern blot analysis, and the presence of CNP was shown by Southern blotting coupled with reverse transcription-polymerase chain reaction (RT-PCR). These results suggest that the CNP/GC-B system is preferentially expressed in ATDC5 cells. GC-B mRNA expression was higher at 14 days after confluency than that at confluency. CNP or 8-bromo cGMP reduced [H-3] thymidine uptake and slightly increased the message for collagen type X. which is a marker of hypertrophic chondrocytes. These data suggest that the CNP/GC-B system is likely to be an autocrine/paracrine regulator of ATDC5 cells, thus affecting both their growth and differentiation.