The ha72 Core Gene of Baculovirus Is Essential for Budded Virus Production and Occlusion-Derived Virus Embedding, and Amino Acid K22 Plays an Important Role in Its Function
The ha72 Core Gene of Baculovirus Is Essential for Budded Virus Production and Occlusion-Derived Virus Embedding, and Amino Acid K22 Plays an Important Role in Its Function
复制标题
杆状病毒ha72核心基因对于芽生病毒产生和包埋衍生病毒包埋至关重要，氨基酸K22在其功能中发挥重要作用
DOI：
10.1128/jvi.02281-13
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发表时间：
2014-01-01
影响因子：
5.4
通讯作者：
Hu, Zhihong
中科院分区：
文献类型：
--
作者：
Huang, Huachao;Wang, Manli;Hu, Zhihong
ABSTRACT ha72 of Helicoverpa armigera nucleopolyhedrovirus (a homologue of ac78) was identified as a conserved late baculovirus gene and characterized. HA72 localizes in the intranuclear ring zone. By generating mutants, we showed that HA72 is essential for budded virus (BD) production and occlusion-derived virus (ODV) embedding. HA72 also interacted with P33, a baculoviral sulfhydryl oxidase. A point mutation of amino acid 22 from lysine to glutamic acid curtailed BV production and precluded ODV occlusion as well as interaction with P33.
