RELATED MANNOSE-SPECIFIC LECTINS FROM DIFFERENT SPECIES OF THE FAMILY AMARYLLIDACEAE
RELATED MANNOSE-SPECIFIC LECTINS FROM DIFFERENT SPECIES OF THE FAMILY AMARYLLIDACEAE
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DOI：
10.1111/j.1399-3054.1988.tb09192.x
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发表时间：
1988-05-01
影响因子：
6.4
通讯作者：
PEUMANS, WJ
中科院分区：
文献类型：
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作者：
VANDAMME, EJM;ALLEN, AK;PEUMANS, WJ
来自石蒜科（Amaryllidaceae）三种植物（水仙（Narcissus narciscissusL.），[医]银合欢和银合欢（Leucojum vernum L.）含有甘露糖特异性凝集素这些凝集素与先前报道的雪花莲（Galanthus nivalisL.）鳞茎，但具有不同的分子结构。本文所描述的凝集素是由13 kDa的亚基组成的二聚体蛋白，它们不通过二硫键连接在一起。在半抗原抑制试验中，石蒜科凝集素对甘露糖表现出排他性特异性。此外，它们与胰蛋白酶处理的兔红细胞均具有高特异性凝集活性，而人红细胞不凝集。
Bulbs from three species of the plant family Amaryllidaceae (Narcissus pseudonurcissusL.,Leucojum aestivumL. andLeucojum vernumL.) were found to contain mannose‐specific lectins. These lectins were serologically identical to a previously reported Amaryllidaceae lectin fromGalanthus nivalisL. bulbs, but had a different molecular structure. The lectins described in this paper are dimeric proteins composed of subunits of 13 kDa, which are not held together by disulphide bridges. In hapten‐inhibition assays Amaryllidaceae lectins exhibited exclusive specificity towards mannose. Furthermore, they all had a high specific agglutination activity with trypsin‐treated rabbit erythrocytes, whereas human red blood cells were not agglutinated.