Identification, expression and chromosome localization of a human gene encoding a novel protein with similarity to the pilB family of transcriptional factors (pilin) and to bacterial peptide methionine sulfoxide reductases
Identification, expression and chromosome localization of a human gene encoding a novel protein with similarity to the pilB family of transcriptional factors (pilin) and to bacterial peptide methionine sulfoxide reductases
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DOI：
10.1016/s0378-1119(99)00131-6
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发表时间：
1999-06-11
期刊：
影响因子：
3.5
通讯作者：
Coca-Prados, M
中科院分区：
文献类型：
--
作者：
Huang, WH;Escribano, J;Coca-Prados, M
本文报道了从人眼睫状体中分离的编码一种新蛋白的消减cDNA（CBS-1）的分离、鉴定和染色体定位。从cDNA的核苷酸序列推断，CBS-1含有由182个氨基酸组成的开放阅读框架，分子量为19.5 kDa。CBS-1与大肠杆菌的ANSA-GAP基因间区（yeaA）中的假定15.5kDa蛋白以及淋病奈瑟氏菌pilB（一种参与pill表达调控的转录因子）的羧基末端区共享显著的核苷酸和氨基酸序列同一性（残基51至182）。有趣的是，CBS-1还与来自幽门螺杆菌和肺炎链球菌的肽-甲硫氨酸亚砜还原酶（MsrA）（一种修复酶）的羧基末端具有显著的同一性。然而，CBS-I的氨基末端（残基23至43）与淋球菌pilB或肺炎球菌MsrA的氨基末端区域缺乏同源性，在20个氨基酸的延伸中与富含甘氨酸的蛋白质表现出显著的同一性。通过北方印迹，CBS-1与大小为0.6至0.7 kb的转录物杂交，在许多组织中普遍表达，但在视网膜和眼睫状体、骨骼肌和心脏中最丰富。针对CBS-1羧基末端氨基酸序列的表位定向抗体识别眼睫状体提取物中19.5kDa的主要蛋白质，以及E. coliMC 1061细胞中高水平表达MsrA。CBS-1基因定位于人染色体10 p12的WI-8535和WI-4724之间，与两个STRP标记D10 S1789和D10 S550紧密连锁。我们建议，CBS-1基因编码的哺乳动物转录因子相关的细菌pilB和某些细菌MsrA同源物。(C)1999 Elsevier Science B. V.保留所有权利。
Here we report the isolation, characterization and chromosome localization of a subtracted cDNA (CBS-1) isolated from the human ocular ciliary body which encodes a novel protein. As is deduced from the nucleotide sequence of the cDNA, CBS-1 contains an open reading frame consisting of 182 amino acids, with a molecular weight of 19.5 kDa. CBS-1 shares significant nucleotide and amino acid sequence identities (residues 51 to 182) with a hypothetical 15.5 kDa protein in the ANSA-GAP intergenic region (yeaA) of Escherichia coli, and the carboxyl terminal region of pilB, a transcription factor involved in the regulation of expression of pill, from Neisseria gonorrhoeae. Interestingly, CBS-1 also shares significant identity with the carboxyl terminus of the peptide-methionine sulfoxide reductase (MsrA), a repair enzyme, from Helicobacter pylori and Streptococcus pneumoniae. However, the amino terminal of CBS-I (residues 23 to 43), which lacks homology to the amino terminal region of gonococcal pilB or pneumococcal MsrA, exhibits significant identity in a stretch of 20 amino acids, with glycine-rich proteins. By Northern blot, CBS-1, hybridized to a 0.6 to 0.7 kb transcript in size, is expressed ubiquitously in many tissues, but most abundantly in the retina and ocular ciliary body, skeletal muscle and heart. An epitope-directed antibody to an amino acid sequence at the carboxyl terminus of CBS-1 recognized a main protein of 19.5 kDa in ocular ciliary body extracts, and a 23 kDa protein in total extracts from E. coli MC1061 cells, which expresses high levels of MsrA. The CBS-1 gene was mapped to human chromosome 10p12 between markers WI-8535 and WI-4724, and is tightly linked to the two STRP markers of D10S1789 and D10S550. We suggest that the CBS-1 gene encodes a mammalian transcription factor related to the bacterial pilB and certain bacterial MsrA homologues. (C) 1999 Elsevier Science B.V. All rights reserved.