MicroRNA (miR)-203 and miR-205 expression patterns identify subgroups of prognosis in cutaneous squamous cell carcinoma.
MicroRNA (miR)-203 and miR-205 expression patterns identify subgroups of prognosis in cutaneous squamous cell carcinoma.
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DOI：
10.1111/bjd.15236
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发表时间：
2017-07
期刊：
影响因子：
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通讯作者：
Pérez-Losada J
中科院分区：
文献类型：
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作者：
Cañueto J;Cardeñoso-Álvarez E;García-Hernández JL;Galindo-Villardón P;Vicente-Galindo P;Vicente-Villardón JL;Alonso-López D;De Las Rivas J;Valero J;Moyano-Sanz E;Fernández-López E;Mao JH;Castellanos-Martín A;Román-Curto C;Pérez-Losada J
皮肤鳞状细胞癌是人类第二大最广泛的癌症，其发病率正在上升。这些肿瘤可以演变为预后不良的疾病，因此重要的是要确定新的标志物，以更好地预测其临床演变。在这里，我们确定了一组小鼠皮肤癌细胞系中皮肤癌进展不同阶段的miRNA表达模式。我们确定了miR-203和miR-205在这组中的差异表达，并评估了它们作为人类肿瘤预后生物标志物的潜在用途。miR-205在具有被认为是预后不良的指标的病理学特征的肿瘤中表达，例如结缔组织增生、神经周围浸润和浸润性生长模式。MiR-205主要表达于未分化区域和浸润前沿，与局部复发和进展不良的一般临床事件的发生相关。选择miR-205表达作为自变量，使用本研究中描述的多项逻辑回归模型预测不良临床进展事件。相比之下，miR-203主要在表现出与良好预后相关的特征的肿瘤中表达，主要存在于分化良好的区域中，并且很少在侵袭前沿表达。因此，miR-205和miR-203的表达和关联大多是相互排斥的。最后，使用逻辑双标图，我们根据miR-203和miR-205表达以及病理肿瘤特征确定了具有不同预后的三组患者。这项工作强调了miR-205和miR-203作为皮肤鳞状细胞癌预后标志物的实用性。
Cutaneous squamous cell carcinoma is the second most widespread cancer in humans and its incidence is rising. These tumours can evolve as poor-prognosis diseases, and therefore it is important to identify new markers to better predict its clinical evolution. Here, we identified the expression pattern of miRNAs at different stages of skin cancer progression in a panel of murine skin cancer cell lines. We determined that miR-203 and miR-205 are differentially expressed in this panel, and evaluated their potential use as biomarkers of prognosis in human tumours. MiR-205 was expressed in tumours with pathological features recognized as indicators of poor prognosis such as desmoplasia, perineural invasion and infiltrative growth pattern. MiR-205 was mainly expressed in undifferentiated areas and in the invasion front, and was associated with both local recurrence and the development of general clinical events of poor evolution. MiR-205 expression was an independent variable selected to predict events of poor clinical evolution using the multinomial logistic regression model described in this study. In contrast, miR-203 was mainly expressed in tumours exhibiting the characteristics associated with a good prognosis, was mainly present in well-differentiated zones, and rarely expressed in the invasion front. Therefore, the expression and associations of miR-205 and miR-203 were mostly mutually exclusive. Finally, using a logistic biplot we identified three clusters of patients with differential prognosis based on miR-203 and miR-205 expression, and pathological tumour features. This work highlights the utility of miR-205 and miR-203 as prognostic markers in cutaneous squamous cell carcinoma.