In vivo and in vitro anti-inflammatory activity of Mangifera indica L. extract (VIMANG®)
In vivo and in vitro anti-inflammatory activity of Mangifera indica L. extract (VIMANG®)
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DOI：
10.1016/j.phrs.2003.12.003
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发表时间：
2004-08-01
影响因子：
9.3
通讯作者：
Delgado, R
中科院分区：
文献类型：
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作者：
Garrido, G;González, D;Delgado, R
在古巴以VIMANG(R)品牌作为抗氧化剂使用的芒果A indica L.的标准水提取物，使用普遍接受的测定法在体内测试了其抗炎活性。外用籼米提取物（0.5 ~ 2 mg /耳）可减轻花生四烯酸（AA）和肉豆蔻酸酯（PMA, ED50 = 1.1 mg /耳）引起的小鼠耳部水肿。在PMA模型中，籼稻提取物还降低了髓过氧化物酶（MPO）活性。在两种炎症模型（AA, ED50 = 106.1 mg kg（-1）和PMA， ED50 = 58.2 mg kg(-1)）中，给予该提取物也抑制了肿瘤坏死因子α (TNFalpha)的血清水平。体外研究采用促炎刺激（LPS-IFNgamma或钙离子载体A23187）刺激巨噬细胞系RAW264.7，分别测定PGE(2)或LTB4的释放。提取物抑制PGE(2)诱导的IC50 = 64.1 μ g ml(-1)，抑制LTB4诱导的IC50 = 22.9 μ g ml（-1）。籼米提取物对人滑膜分泌磷脂酶（PL）A(2)也有抑制作用，IC50 = 0.7马克杯ml（-1）。这些结果为阐明标准籼稻提取物VMANG(R)的抗炎和抗伤害作用的机制做出了重要贡献。(C) 2004 Elsevier Ltd.版权所有。
A standard aqueous extract of Mangifera indica L., used in Cuba as an antioxidant under the brand name of VIMANG(R), was tested in vivo for its anti-inflammatory activity using commonly accepted assays. M. indica extract, administered topically (0.5-2 mg per ear), reduced ear edema induced by arachidonic acid (AA) and phorbol myristate acetate (PMA, ED50 = 1.1 mg per ear) in mice. In the PMA model, M. indica extract also reduced myeloperoxidase (MPO) activity. This extract p.o. administered also inhibited tumor necrosis factor alpha (TNFalpha) serum levels in both models of inflammation (AA, ED50 = 106.1 mg kg(-1) and PMA, ED50 = 58.2 mg kg(-1)). In vitro studies were performed using the macrophage cell line RAW264.7 stimulated with pro-inflammatory stimuli (LPS-IFNgamma or the calcium ionophore A23187) to determine PGE(2) or LTB4 release, respectively. The extract inhibited the induction of PGE(2) with IC50 = 64.1 mug ml(-1) and LTB4 IC50 = 22.9 mug ml(-1). M. indica extract also inhibited human synovial secretory phospholipase (PL)A(2) with IC50 = 0.7 mug ml(-1). These results represent an important contribution to the elucidation of the mechanism involved in the anti-inflammatory and anti-nociceptive effects reported by the standard M. indica extract VMANG(R). (C) 2004 Elsevier Ltd. All rights reserved.