Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation.
Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation.
复制标题
尖端技术：DNA 聚合酶 mu 和 lambda 对于 Ig 基因超突变来说是可有可无的。
作者：
B. Bertocci;A. De Smet;E. Flatter;A. Dahan;J. Bories;C. Landreau;J. Weill;C. Reynaud
免疫应答过程中Ig V基因的突变很可能是由一个或几个容易出错的DNA聚合酶引起的。许多最近描述的非复制性DNA聚合酶具有与这种活性相容的内在保真度，最强的候选是聚合酶（pol） eta, pol iota， pol zeta和pol mu。我们在这项工作中报告说，灭活与pol β（即pol mu和pol lambda）相关的两种聚合酶中的任何一种的小鼠是活的和可生育的，并显示出正常的超突变模式。
Mutations arising in Ig V genes during an immune response are most likely introduced by one or several error-prone DNA polymerases. Many of the recently described nonreplicative DNA polymerases have an intrinsic fidelity compatible with such an activity, the strongest candidates being polymerase (pol) eta, pol iota, pol zeta, and pol mu. We report in this work that mice inactivated for either of the two polymerases related to pol beta (i.e., pol mu and pol lambda) are viable and fertile and display a normal hypermutation pattern.