GhLTPG1, a cotton GPI-anchored lipid transfer protein, regulates the transport of phosphatidylinositol monophosphates and cotton fiber elongation.
GhLTPG1, a cotton GPI-anchored lipid transfer protein, regulates the transport of phosphatidylinositol monophosphates and cotton fiber elongation.
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GhLTPG1 是一种棉花 GPI 锚定的脂质转移蛋白，调节磷脂酰肌醇单磷酸的运输和棉纤维伸长
DOI：
10.1038/srep26829
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发表时间：
2016-06-17
影响因子：
4.6
通讯作者：
Zuo K
中科院分区：
文献类型：
--
作者：
Deng T;Yao H;Wang J;Wang J;Xue H;Zuo K
棉纤维是从胚珠表皮延伸出来的种子毛状体。极性脂质是细胞膜快速扩大和纤维细胞生长所必需的，然而，脂质是如何从胚珠运输到发育中的纤维的，目前尚不清楚。在这里，我们报道了GhLTPG1（一种gpi锚定的脂质转运蛋白）在棉纤维伸长过程中的功能特征。GhLTPG1蛋白在棉纤维和胚珠外被中大量表达，位于细胞膜上。生化分析表明，GhLTPG1在体外特异性结合磷脂肌醇单磷酸（PtdIns3P、PtdIns4P和PtdIns5P），并在体内将PtdInsPs从合成部位转运到质膜。在拟南芥中表达ghltpg1导致毛状体和纤维数量增加。敲低ghltpg1的棉花植株表现出显著的长度减少、极性脂含量降低和纤维伸长相关基因表达抑制。这些结果表明，GhLTPG1蛋白通过介导单磷酸磷脂酰肌醇的转运来调控棉纤维伸长。
The cotton fibers are seed trichomes that elongate from the ovule epidermis. Polar lipids are required for the quick enlargement of cell membrane and fiber cell growth, however, how lipids are transported from the ovules into the developing fibers remains less known. Here, we reported the functional characterization of GhLTPG1, a GPI-anchored lipid transport protein, during cotton fiber elongation.GhLTPG1was abundantly expressed in elongating cotton fibers and outer integument of the ovules and GhLTPG1 protein was located on cell membrane. Biochemical analysis showed that GhLTPG1 specifically bound to phosphatidylinositol mono-phosphates (PtdIns3P, PtdIns4P and PtdIns5P)in vitroand transported PtdInsPs from the synthesis places to the plasma membranesin vivo. Expression ofGhLTPG1in Arabidopsis caused an increased number of trichomes and fibers inGhLTPG1-knockdown cotton plants exhibited significantly reduced length, decreased polar lipid content and repression of fiber elongation-related genes expression. These results suggested that GhLTPG1 protein regulates the cotton fiber elongation through mediating the transport of phosphatidylinositol monophosphates.