Spatiotemporal patterns of Musashi1 expression during inner ear development
Spatiotemporal patterns of Musashi1 expression during inner ear development
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内耳发育过程中 Musashi1 表达的时空模式
DOI：
10.1097/00001756-200404290-00013
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发表时间：
2004
期刊：
影响因子：
1.7
通讯作者：
S. Fushiki
中科院分区：
文献类型：
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作者：
H. Sakaguchi;T. Yaoi;Toshihiro Suzuki;H. Okano;Y. Hisa;S. Fushiki
Musashi1(MSI 1)是一种与神经前体细胞不对称分裂相关的RNA结合蛋白。为探讨Msi1基因在内耳发育中的作用，采用RT-PCR和免疫组织化学方法检测了Msi1基因在小鼠内耳组织中的表达。免疫组织化学显示Msi1在胚胎第10天和第12天在所有耳囊细胞中均有表达。Msi1免疫反应在胚胎14天后在前庭毛细胞中消失，在16天后在耳蜗毛细胞中消失，但在成年后一直维持在支持细胞中。在出生后2周，Msi1的亚细胞定位由“胞浆优势”转变为“核优势”。提示MSI在内耳发育中可能起一定作用。
Musashi1 (Msi 1) is an RNA binding protein associated with asymmetric cell divisions in neural progenitor cells. To investigate the involvement of Msi1 in the inner ear development, we studied the expression of Msi1 in mouse inner ears with RT-PCR and immunohistochemistry. Immunohistochemistry revealed that Msi1 was expressed in all otocyst cells at embryonic day (E) 10 and 12. Msi1 immunoreactivity became lost in hair cells after E14 in vestibule and after E16 in cochlea, whereas it persisted in supporting cells until adulthood. The subcellular localization of Msi1 changed from “cytoplasmic predominance” to “nuclear predominance” during the first 2 weeks after birth. The present data suggested that Msi may play a role in inner ear development.
Math1 在内耳发育中的作用：将感觉原基的建立与毛细胞命运的决定分开。
DOI：
10.1242/dev.129.10.2495
发表时间：
2002
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Chen,Ping;Johnson,JaneE;Zoghbi,HudaY;Segil,Neil
通讯作者：
Segil,Neil