In situ monitoring of intracellular controlled drug release from mesoporous silica nanoparticles coated with pH-responsive charge-reversal polymer.
In situ monitoring of intracellular controlled drug release from mesoporous silica nanoparticles coated with pH-responsive charge-reversal polymer.
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DOI：
10.1021/am5059519
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发表时间：
2014-10
影响因子：
9.5
通讯作者：
P. Zhang;Tong Wu;J. Kong
中科院分区：
文献类型：
--
作者：
P. Zhang;Tong Wu;J. Kong
响应物理和生物刺激的治疗平台如化疗对于有效的癌症治疗是高度期望的。在这项研究中，pH响应的电荷反转，聚合物包覆的介孔二氧化硅纳米粒子[PAH-cit/APTES-MSNs; PAH-cit是指聚（烯丙基胺）-柠康酸酐; APTES是指（3-氨丙基）三乙氧基硅烷]的合成应用程序作为药物递送系统用于治疗恶性细胞。共聚焦激光扫描显微镜（CLSM）显示PAH-cit/APTES-MSNs纳米复合物有效地将盐酸阿霉素递送并释放到HeLa（人宫颈癌）细胞核。此外，通过CLSM实时动态监测药物释放过程。目前的pH控制智能释放平台在药物递送和癌症治疗相关应用中具有前景。
Therapeutic platforms such as chemotherapy that respond to physical and biological stimuli are highly desirable for effective cancer therapy. In this study, pH-responsive charge-reversal, polymer-coated mesoporous silica nanoparticles [PAH-cit/APTES-MSNs; PAH-cit refers to poly(allylamine)-citraconic anhydride; APTES refers to (3-aminopropyl)triethoxysilane] were synthesized for application as drug-delivery systems for the treatment of malignant cells. Confocal laser scanning microscopy (CLSM) revealed that the PAH-cit/APTES-MSNs nanocomposite effectively delivered and released doxorubicin hydrochloride to the nucleus of HeLa (human cervical carcinoma) cells. Additionally, the real-time dynamic drug-release process was monitored by CLSM. The current pH-controlled-smart-release platform holds promise in drug-delivery and cancer therapy-related applications.