Cellular correlate of assembly formation in oscillating hippocampal networks in vitro
Cellular correlate of assembly formation in oscillating hippocampal networks in vitro
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DOI：
10.1073/pnas.1103546108
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发表时间：
2011-08-30
影响因子：
11.1
通讯作者：
Draguhn, Andreas
中科院分区：
文献类型：
--
作者：
Baehner, Florian;Weiss, Elisa K.;Draguhn, Andreas
神经元形成短暂稳定的组装，这可能是认知功能（包括记忆形成）的基础。在大多数大脑区域，相干活动是由网络振荡组织的，网络振荡涉及在定义明确的少数细胞内的稀疏放电。尽管对潜在的细胞机制进行了大量的研究，但一个基本问题仍然没有解决：如何将参与神经元与大多数非参与神经元区分开来？我们使用生理和建模技术来分析自发发生的高频网络振荡过程中小鼠海马脑片的神经元活动。网络夹带的动作电位只观察到一个定义的锥体细胞的子集，严格区分参与和nonparticipating神经元。这些尖峰具有独特的性质，因为它们是在轴突中产生的，而没有预先对索马进行去极化。GABA（A）受体在锥体细胞募集中具有双重作用。首先，稀疏的夹带穗的发生是通过强烈的体周抑制来完成的。第二，逆行锋电位的产生促进了GABA的紧张作用在远程轴突室。异位峰生成连同强烈的体树突抑制可能提供了一个细胞机制的振荡组件的定义。
Neurons form transiently stable assemblies that may underlie cognitive functions, including memory formation. In most brain regions, coherent activity is organized by network oscillations that involve sparse firing within a well-defined minority of cells. Despite extensive work on the underlying cellular mechanisms, a fundamental question remains unsolved: how are participating neurons distinguished from the majority of nonparticipators? We used physiological and modeling techniques to analyze neuronal activity in mouse hippocampal slices during spontaneously occurring high-frequency network oscillations. Network-entrained action potentials were exclusively observed in a defined subset of pyramidal cells, yielding a strict distinction between participating and nonparticipating neurons. These spikes had unique properties, because they were generated in the axon without prior depolarization of the soma. GABA(A) receptors had a dual role in pyramidal cell recruitment. First, the sparse occurrence of entrained spikes was accomplished by intense perisomatic inhibition. Second, antidromic spike generation was facilitated by tonic effects of GABA in remote axonal compartments. Ectopic spike generation together with strong somatodendritic inhibition may provide a cellular mechanism for the definition of oscillating assemblies.