DROSOPHILA DOUBLESEX GENE CONTROLS SOMATIC SEXUAL-DIFFERENTIATION BY PRODUCING ALTERNATIVELY SPLICED MESSENGER-RNAS ENCODING RELATED SEX-SPECIFIC POLYPEPTIDES
DROSOPHILA DOUBLESEX GENE CONTROLS SOMATIC SEXUAL-DIFFERENTIATION BY PRODUCING ALTERNATIVELY SPLICED MESSENGER-RNAS ENCODING RELATED SEX-SPECIFIC POLYPEPTIDES
复制标题
DOI：
10.1016/0092-8674(89)90633-8
复制
发表时间：
1989-03-24
期刊：
影响因子：
64.5
通讯作者：
BAKER, BS
中科院分区：
文献类型：
--
作者：
BURTIS, KC;BAKER, BS
双性 (dsx) 基因调节黑腹果蝇两性的体细胞性别分化。 dsx编码两种功能产物：一种产物在雌性中表达并抑制雄性分化，另一种在雄性中表达并抑制雌性分化。我们已经确定 dsx 基因被转录产生共同的初级转录物，该转录物被选择性剪接和多聚腺苷酸化以产生男性和女性特异性 mRNA。这些性别特异性mRNA共享一个共同的5''末端和三个共同的外显子，但具有替代的性别特异性3''外显子，因此编码具有共同氨基末端序列但性别特异性羧基末端的多肽。遗传和分子数据表明，包括和邻近雌性特异性剪接受体位点的序列在transformer和transformer-2基因座调节dsx表达中发挥重要作用。
The doublesex (dsx) gene regulates somatic sexual differentiation in both sexes in D. melanogaster. Two functional products are encoded by dsx: one product is expressed in females and represses male differentiation, and the other is expressed in males and represses female differentiation. We have determined that the dsx gene is transcribed to produce a common primary transcript that is alternatively spliced and polyadenylated to yield male- and female-specific mRNAs. These sex-specific mRNAs share a common 5'' end and three common exons, but possess alternative sex-specific 3'' exons, thus encoding polypeptides with a common amino-terminal sequence but sex-specific carboxyl termini. Genetic and molecular data suggest that sequences including and adjacent of the female-specific splice acceptor site play an important role in the regulation of dsx expression by the transformer and transformer-2 loci.