Scheduled exercise phase shifts the circadian clock in skeletal muscle.
Scheduled exercise phase shifts the circadian clock in skeletal muscle.
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DOI：
10.1249/mss.0b013e318255cf4c
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发表时间：
2012-09
影响因子：
4.1
通讯作者：
Esser KA
中科院分区：
文献类型：
--
作者：
Wolff G;Esser KA
在哺乳动物中，昼夜节律行为和分子时钟可以通过下丘脑的视交叉上核(SCN)与光-暗(LD)周期同步。除了光，已经证明了非光时间线索，如限制食物可获得的时间，可以改变昼夜行为和特定外周组织(如肝脏)的时钟基因表达。研究还表明，有计划的体力活动(锻炼)可以改变行为和时钟基因表达的昼夜节律，但目前单独锻炼的影响在很大程度上是未知的，也没有在骨骼肌中进行探索。PER2∷荧光素酶(PER2∷LUC)小鼠在光照12h和黑暗12h(12L：12D)下维持，然后在光期进行2小时的自愿或非自愿运动，共4周。对照组小鼠被留在家中的笼子里或被转移到运动环境(Sham)。第二组小鼠限制进食(每天4小时，持续2周)，以比较两种非光线索对PER2∷LUC生物发光的影响。取骨骼肌、肺和SCN组织块培养5~6d，研究分子节律。在运动小鼠的骨骼肌和肺组织中，PER2∷LUC生物发光峰的峰位移，而SCN则没有，这表明运动对周围组织中的分子时钟具有特异性的同步性。这些数据提供了证据，表明外周组织中的分子生物钟可以对运动时间做出反应，这表明体力活动为整个身体的生物钟同步提供了重要的计时信息。
It has been well established in mammals that circadian behavior as well as the molecular clockwork can be synchronized to the light-dark (LD) cycle via the suprachiasmatic nucleus of the hypothalamus (SCN). In addition to light, it has been demonstrated that non-photic time cues, such as restricting the time of food availability, can alter circadian behavior and clock gene expression in selected peripheral tissues such as liver. Studies have also suggested that scheduled physical activity (exercise) can alter circadian rhythms in behavior and clock gene expression, however currently the effects of exercise alone are largely unknown and have not been explored in skeletal muscle. Period2∷Luciferase (Per2∷Luc) mice were maintained under 12 hours of light followed by 12 hours of darkness (12L:12D) then exposed to 2 hours of voluntary or involuntary exercise during the light phase for 4 weeks. Control mice were left in home cages or moved to the exercise environment (sham). A second group of mice had restricted access to food (4 hours per day for 2 weeks) in order to compare the effects of two non-photic cues on PER2∷LUC bioluminescence. Skeletal muscles, lung and SCN tissue explants were cultured for 5-6 days to study molecular rhythms. In the exercised mice, the phase of peak PER2∷LUC bioluminescence was shifted in the skeletal muscle and lung explants but not the SCN suggesting a specific synchronizing effect of exercise on the molecular clockwork in peripheral tissues. These data provide evidence that the molecular circadian clock in peripheral tissues can respond to the time of exercise suggesting that physical activity contributes important timing information for synchronization of circadian clocks throughout the body.