Spatial dynamics of intracellular calcium in agonist-stimulated vascular smooth muscle cells.
Spatial dynamics of intracellular calcium in agonist-stimulated vascular smooth muscle cells.
复制标题
激动剂刺激的血管平滑肌细胞中细胞内钙的空间动力学。
DOI：
--
复制
发表时间：
1990
影响因子：
--
通讯作者：
R. Irvine
中科院分区：
文献类型：
--
作者：
C. Neylon;J. Hoyland;W. Mason;R. Irvine
血管收缩剂激动剂通过诱导细胞内游离Ca 2+升高来刺激平滑肌收缩。从人内乳动脉培养的单个血管平滑肌细胞的fura-2荧光的数字成像显微镜使我们能够记录受体激动剂刺激后立即发生的Ca 2+亚细胞变化。凝血酶诱导的细胞质游离Ca 2+的升高开始于通常位于细胞末端附近的离散区域。随后，该升高的Ca 2+区域扩展，直到整个细胞质中的Ca 2+升高。在Ca 2+升高的区域中沿线性方向的扩散速率平均为10.1微米/秒，使其能够在约5秒内穿过大多数细胞的长度，并且涉及200至500 nM之间的Ca 2+升高。在一些细胞中，Ca 2+的上升从两端开始，并在中途发生碰撞。在乙酰胆碱刺激的细胞中记录到类似的动态变化的Ca 2+的空间分布。血管收缩剂激动剂诱导局部区域的Ca 2+升高，随后在单个平滑肌细胞的整个细胞质中扩展，这一新的观察结果可能为血管组织中Ca 2+信号转导的性质提供新的见解。
Vasoconstrictor agonists stimulate smooth muscle contraction by inducing a rise in intracellular free Ca2+. Digital-imaging microscopy of fura-2 fluorescence from single vascular smooth muscle cells cultured from the human internal mammary artery has allowed us to record the subcellular alterations in Ca2+ that occur immediately after stimulation by receptor agonists. The thrombin-induced rise in cytoplasmic free Ca2+ begins in a discrete region typically located close to the end of the cell. Subsequently, this region of elevated Ca2+ expands until Ca2+ is elevated throughout the cell cytoplasm. The rate of spreading in the region of elevated Ca2+ in a linear direction averaged 10.1 microns/s, enabling it to traverse the length of most cells within approximately 5 s, and involved rises in Ca2+ of between 200 and 500 nM. In some cells, the Ca2+ rise began at both ends and collided midway. Similar dynamic changes in the spatial distribution of Ca2+ were recorded in cells stimulated by acetylcholine. The novel observation that vasoconstrictor agonists induce an elevation of Ca2+ in a localized region which subsequently expands throughout the cytoplasm of single smooth muscle cells may provide new insight into the nature of Ca2+ signaling in vascular tissue.
分离的胃平滑肌细胞中肌醇 1,4,5-三磷酸的收缩和 Ca2 动员的化学计量。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bitar,KN;Bradford,PG;PutneyJr,JW;Makhlouf,GM
通讯作者：
Makhlouf,GM