Abortive initiation and long ribonucleic acid synthesis.
Abortive initiation and long ribonucleic acid synthesis.
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失败的起始和长核糖核酸合成。
DOI：
10.1021/bi00511a003
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Reznikoff,WS
中科院分区：
文献类型：
--
作者：
Munson,LM;Reznikoff,WS
材料和方法从Sigma中获得sheparin和未标记的三磷酸核苷。[a-32P] UTP来自Amers-ham （J> 350 Ci/mmol）。Bis (acrylycystamine)（BAC）购自Bio-Rad Laboratories。RNA聚合酶是L. Maquat和S. Rothstein的礼物。从New England biabs或Bethesda Research Labs inc .获得限制性内切酶，用HaeIII酶切从pRZ3（野生型启动子）、pRZ3111 （Prl 11; Maquat etal., 1980）和pRZ3UV5 （L8UV5）中分离出含有乳糖控制区的203碱基对限制性内切片段（bp）[质粒结构，见Hardies etal]。（1979）和Maquat & Rez-nikoff(1978)]。转座子Tn5启动子分离得到
Materials and MethodsHeparin and unlabeled nucleoside triphosphates were ob-tained from Sigma.[a-32P] UTP was obtained from Amers-ham (J> 350 Ci/mmol). Bis (acrylylcystamine)(BAC) was purchased from Bio-Rad Laboratories. RNA polymerase was a gift from L. Maquat and S. Rothstein. Restriction enzymes were obtained from either New England Biolabs or Bethesda Research Labs Inc.The 203-base-pair (bp) restriction fragment containing the lactose control region was isolated from pRZ3 (wild-type promoter), pRZ3111 (Prl 11; Maquat et al., 1980), and pRZ3UV5 (L8UV5) by digestion with HaeIII [for plasmid constructions, see Hardies etal.(1979) and Maquat & Rez-nikoff (1978)]. The transposon Tn5 promoter was isolated