Association of the C677T Polymorphism in the Human Methylenetetrahydrofolate Reductase (MTHFR) Gene with the Genetic Predisposition for Type 2 Diabetes Mellitus in a Moroccan Population
Association of the C677T Polymorphism in the Human Methylenetetrahydrofolate Reductase (MTHFR) Gene with the Genetic Predisposition for Type 2 Diabetes Mellitus in a Moroccan Population
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DOI：
10.1089/gtmb.2011.0179
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发表时间：
2012-05-01
影响因子：
1.4
通讯作者：
Barakat, Abdelhamid
中科院分区：
文献类型：
--
作者：
Benrahma, Houda;Abidi, Omar;Barakat, Abdelhamid
目的：2型糖尿病（T2 DM）是全球主要的公共卫生问题。亚甲基四氢叶酸还原酶（MTHFR）基因C677 T和A1298 C多态性与2型糖尿病及其并发症相关。本研究旨在调查摩洛哥人口中的这种关联。方法：对282例摩洛哥糖尿病患者和232例健康对照者进行病例对照研究。MTHFR C677 T和A1298 C多态性通过聚合酶链反应进行基因分型，然后分别用HinfI和MboII酶进行酶消化。结果：C677 T基因多态性在加性和显性模型中均与2型糖尿病有显著相关性。此外，677 T等位基因频率在糖尿病组和对照组之间存在显著差异（分别为26.06%和33.20%）。然而，未发现A1298 C多态性与T2 DM之间存在显着相关性。677 CC/1298 AA和677 CT/1298 AC基因型的频率在糖尿病组和对照组之间差异显著（分别为32.62%和20.61%; 9.57%和17.55%）。结论：这些结果表明MTHFR C677 T多态性与摩洛哥T2 DM患者之间存在明显关联，但与MTHFR A1298 C多态性无显著关联。
Aims: Type 2 diabetes mellitus (T2DM) is a major public health problem around the world. The C677T and A1298C polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) gene have been reported to be associated with T2DM and its complications. This study aimed to investigate this association in the Moroccan population. Methods: A case-control study was performed among 282 Moroccan diabetic patients and 232 healthy controls. The MTHFR C677T and A1298C polymorphisms were genotyped by polymerase chain reaction, followed by enzymatic digestion with HinfI and MboII enzymes, respectively. Results: There was a significant association between C677T polymorphism and T2DM in both additive and dominant models. In addition, the 677T allele frequency differed significantly between the diabetic and control groups (26.06% vs. 33.20%, respectively). However, no significant association was found between A1298C polymorphism and T2DM. The frequencies of combined genotypes 677CC/1298AA and 677CT/1298AC differed significantly between the diabetic and control groups (32.62% vs. 20.61% and 9.57% vs. 17.55%, respectively). Conclusions: These results show an evident association between the MTHFR C677T polymorphism and T2DM in Moroccan patients but no significant association with the MTHFR A1298C polymorphism.