New and effective entrapment of polyelectrolyte-enzyme-complexes in LentiKats
New and effective entrapment of polyelectrolyte-enzyme-complexes in LentiKats
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DOI：
10.1023/a:1010569322537
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发表时间：
2001-08-01
影响因子：
2.7
通讯作者：
Vorlop, KD
中科院分区：
文献类型：
--
作者：
Czichocki, G;Dautzenberg, H;Vorlop, KD
淀粉葡糖苷酶EC 3.2.1.3用作在聚（乙烯醇）水凝胶（LentiKats）镜片中固定酶的模型。PVA-水凝胶中的酶的包埋基于两步程序，首先其偶联到聚电解质增加了酶的结构水平，随后，所得复合物被包埋在LentiKats中。固定化的酶保留了45%的原始活性，并在五个重复的批次运行中没有失去活性。
Amyloglucosidase EC 3.2.1.3 was used as model for the immobilisation of enzymes in poly(vinylalcohol) hydrogel (LentiKats) lenses. The entrapment of the enzyme in PVA-hydrogel based on a two-step procedure, firstly its coupling to polyelectrolytes increased the structure level of the enzyme, and subsequently, the resulting complex was entrapped in LentiKats. The immobilised enzyme retained 45% of its original activity and lost no activity over five repeated batch runs.