Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles
Fibre-infused gel scaffolds guide cardiomyocyte alignment in 3D-printed ventricles
复制标题
DOI：
10.1038/s41563-023-01611-3
复制
发表时间：
2023-07-27
期刊：
影响因子：
41.2
通讯作者：
Parker, Kevin Kit
中科院分区：
文献类型：
--
作者：
Choi, Suji;Lee, Keel Yong;Parker, Kevin Kit
水凝胶是用于组织工程的有吸引力的材料，但是迄今为止的努力已经显示出有限的能力来产生促进细胞自组织成分层三维（3D）器官模型所必需的微观结构特征。在这里，我们开发了一种含有预制明胶纤维的水凝胶墨水，用于打印3D器官水平的支架，这些支架重现了心脏的细胞内和细胞间组织。将预制明胶纤维添加到水凝胶中能够定制油墨流变性，从而允许受控的溶胶-凝胶转变，以在没有额外支撑材料的情况下实现独立式3D结构的精确打印。在油墨挤出过程中，剪切诱导的纤维排列提供了微尺度的几何线索，促进体外培养的人心肌细胞自组织成各向异性的肌肉组织。由此产生的3D打印心室体外模型表现出仿生各向异性的电生理和收缩特性。明胶-藻酸盐水凝胶墨水包含短明胶纤维，引导人类心肌细胞自组织成可收缩的组织，这些组织可以3D打印成模仿人体器官的结构。
Hydrogels are attractive materials for tissue engineering, but efforts to date have shown limited ability to produce the microstructural features necessary to promote cellular self-organization into hierarchical three-dimensional (3D) organ models. Here we develop a hydrogel ink containing prefabricated gelatin fibres to print 3D organ-level scaffolds that recapitulate the intra- and intercellular organization of the heart. The addition of prefabricated gelatin fibres to hydrogels enables the tailoring of the ink rheology, allowing for a controlled sol-gel transition to achieve precise printing of free-standing 3D structures without additional supporting materials. Shear-induced alignment of fibres during ink extrusion provides microscale geometric cues that promote the self-organization of cultured human cardiomyocytes into anisotropic muscular tissues in vitro. The resulting 3D-printed ventricle in vitro model exhibited biomimetic anisotropic electrophysiological and contractile properties.A gelatin-alginate hydrogel ink incorporating short gelatin fibres guides the self-organization of human cardiomyocytes into contractile tissues that can be 3D-printed into structures mimicking human organs.