Chemiluminescence cloth-based glucose test sensors (CCGTSs): A new class of chemiluminescence glucose sensors
Chemiluminescence cloth-based glucose test sensors (CCGTSs): A new class of chemiluminescence glucose sensors
复制标题
化学发光布基葡萄糖测试传感器 (CCGTS)：一类新型化学发光葡萄糖传感器
DOI：
10.1016/j.bios.2016.12.004
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发表时间：
2017-05-15
影响因子：
12.6
通讯作者：
Zhang, Chunsun
中科院分区：
文献类型：
--
作者：
Li, Huijie;Liu, Cuiling;Zhang, Chunsun
化学发光在许多领域有着广泛的应用，但目前还没有一种简单、经济、有效的方法。在这项工作中，第一次，CL布基葡萄糖测试传感器（CCGTS）被开发为一类新的CL葡萄糖传感器，不需要复杂，昂贵的器件制造和外围设备。当在流动通道中与期望的疏水屏障和由加载区和检测区之间的高度差引起的重力/毛细流动集成时，单个基于布的装置可以执行包括两个酶反应步骤的整个CL过程。蜡丝网印刷的方法是用来制造超便宜的CCGTS，葡萄糖检测包括葡萄糖的酶促氧化为葡萄糖酸和H2 O2，然后由辣根过氧化物酶（HRP）催化氧化鲁米诺由H2 O2，和发射的CL信号提高与p-碘苯酚（PIP）和成像使用一个廉价的，便携式CCD相机。在优化的条件下，葡萄糖的测定范围为0.1-100 mM，检测限为0.0948 mM，分析时间小于5.5 min。最后，证明了CCGTS用于临床尿液和血清中葡萄糖测量的适用性和有效性。样本。因此，所提出的传感器可以在许多领域的应用中提供很大的希望，并且可以促进即时检测的实现。
Chemiluminescence (CL) has been widely applied in many fields, but it is rarely used in a very simple, economical but effective way. In this work, for the first time, the CL cloth-based glucose test sensors (CCGTSs) are developed as a new class of CL glucose sensors, with no need for complicated, expensive device fabrication and peripheral equipment. When integrated with desirable hydrophobic barrier in the flow channel and gravity/ capillary flow induced by a difference in height between the loading zone and the detection zone, a single cloth based device can perform the whole CL process involving two steps of enzyme reactions. The wax screen printing approach is used to fabricate ultra-cheap CCGTSs, the glucose detection involves the enzymatic oxidation of glucose to gluconic acid and H2O2 followed by the horseradish peroxidase (HRP)-catalyzed oxidation of luminol by H2O2, and the emitted CL signals are heightened with p-iodophenol (PIP) and imaged using an inexpensive, portable CCD camera. Under optimized conditions, glucose can be determined over the range of 0.1-100 mM, with a detection limit of 0.0948 mM and an analysis time of less than 5.5 min. Finally, the applicability and validity of the CCGTSs are demonstrated for the measurement of glucose in clinical urine and serum samples. Thus, the proposed sensors could provide great promise in applications in many areas, and may facilitate the achievement of point-of-care testing.