Thiol synthesis and arsenic hyperaccumulation in Pteris vittata (Chinese brake fern).
Thiol synthesis and arsenic hyperaccumulation in Pteris vittata (Chinese brake fern).
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DOI：
10.1016/j.envpol.2004.03.010
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发表时间：
2004-10
影响因子：
8.9
通讯作者：
Weihua Zhang;Yong Cai;K. Downum;L. Ma
中科院分区：
文献类型：
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作者：
Weihua Zhang;Yong Cai;K. Downum;L. Ma
摘要马齿蕨在砷污染场地的植物修复中具有潜在的应用价值。研究了砷暴露条件下，杜仲中总硫醇和酸溶性硫醇的合成。植物叶片中As浓度与酸溶性硫醇之间的强正相关性表明，酸溶性硫醇可能在As解毒中发挥作用。一个主要的砷诱导的硫醇进行纯化和表征。540 m/z的分子离子（M+1）表明硫醇是具有两个碱基单元（PC 2）的植物螯合素（PC）。然而，酸溶性硫醇的比例，以作为在小叶暴露于As的范围从0.012到0.026，这表明只有很小的一部分作为络合的PC 2。PC在这种超富集植物中可能起着轻微的解毒作用。非PC依赖性机制似乎主要参与As耐受性，而PC依赖性解毒机制似乎是一种补充。
Pteris vittata (Chinese brake fern) has potential for phytoremediation of As-contaminated sites. In this study, the synthesis of total thiols and acid-soluble thiols in P. vittata was investigated under arsenic exposure. The strong and positive correlation between As concentration and acid-soluble thiols in plant leaflets suggests that acid-soluble thiols may play a role in As detoxification. A major As-induced thiol was purified and characterized. A molecular ion (M+1) of 540 m/z suggests that the thiol was a phytochelatin (PC) with two base units (PC2). However, the ratios of acid-soluble thiols to As in leaflets exposed to As ranged from 0.012 to 0.026, suggesting that only a very small part of As is complexed by PC2. PCs could play a minor detoxification role in this hyperaccumulator. A PC-independent mechanism appears to be mainly involved in As tolerance, while PC-dependent detoxification seems to be a supplement.