Widely targeted metabolome and transcriptome landscapes of Allium fistulosum-A. cepa chromosome addition lines revealed a flavonoid hot spot on chromosome 5A
Widely targeted metabolome and transcriptome landscapes of Allium fistulosum-A. cepa chromosome addition lines revealed a flavonoid hot spot on chromosome 5A
复制标题
DOI：
10.1038/s41598-019-39856-1
复制
发表时间：
2019-03-05
影响因子：
4.6
通讯作者：
Shigyo, Masayoshi
中科院分区：
文献类型：
--
作者：
Abdelrahman, Mostafa;Hirata, Sho;Shigyo, Masayoshi
在这里，我们报告了对具有葱 [A. cepa L. Aggregatum group (AA)]阐明类黄酮生物合成中的新基因功能。使用选定的液相色谱-串联四极杆质谱反应监测模式进行了详尽的代谢组学分析，揭示了 AA 和 FF5A 中槲皮素、花青素和黄酮糖苷的特定积累。小葱向A. fistulosum添加的染色体5A诱导了受体物种中类黄酮的积累，这与几个基因的上调有关，包括二氢黄酮醇4-还原酶、查尔酮合酶、黄酮3-羟化酶、UDP-葡萄糖类黄酮-3-O-葡萄糖基转移酶、花青素5-芳香酰基转移酶样、多效性耐药样ATP 结合盒转运蛋白和 MYB14 转录因子。此外，通过使用来自不同遗传种群（包括A. fistulosum-A）的RNA-Seq数据，生成了开放获取的葱转录数据库（Allium TDB，http://alliumtdb.kazusa.or.jp）。 cepa单体加成系。这里提出的功能基因组方法提供了一种针对洋葱中负责类黄酮生物合成的基因的创新方法。对类黄酮化合物和生物合成相关基因的了解将有助于开发具有独特化学成分的高贵葱属品种，从而提高植物的抗逆性和人类健康益处。
Here, we report a comprehensive analysis of the widely targeted metabolome and transcriptome profiles of Allium fistulosum L. (FF) with the single extra chromosome of shallot [A. cepa L. Aggregatum group (AA)] to clarify the novel gene functions in flavonoid biosynthesis. An exhaustive meta bolome analysis was performed using the selected reaction monitoring mode of liquid chromatography-tandem quadrupole mass spectrometry, revealing a specific accumulation of quercetin, anthocyanin and flavone glucosides in AA and FF5A. The addition of chromosome 5A from the shallot to A. fistulosum induced flavonoid accumulation in the recipient species, which was associated with the upregulation of several genes including the dihydrofiavonol 4-reductase, chalcone synthase, fiavanone 3-hydroxylase, UDP-glucose flavonoid-3-O-glucosyltransferase, anthocyanin 5-aromatic acyltransferase-like, pleiotropic drug resistance-like ATP binding cassette transporter, and MYB14 transcriptional factor. Additionally, an open access Allium Transcript Database (Allium TDB, http://alliumtdb.kazusa.or.jp) was generated by using RNA-Seq data from different genetic stocks including the A. fistulosum-A. cepa monosomic addition lines. The functional genomic approach presented here provides an innovative means of targeting the gene responsible for flavonoid biosynthesis in A. cepa. The understanding of flavonoid compounds and biosynthesis-related genes would facilitate the development of noble Allium varieties with unique chemical constituents and, subsequently, improved plant stress tolerance and human health benefits.