A comparative study of the virulence of mycobacterium tuberculosis measured in mice and guinea pigs.
A comparative study of the virulence of mycobacterium tuberculosis measured in mice and guinea pigs.
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在小鼠和豚鼠中测量的结核分枝杆菌毒力的比较研究。
DOI：
10.1164/arrd.1969.100.5.631
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
M. M. Smith
中科院分区：
文献类型：
--
作者：
F. Collins;M. M. Smith
将结核分枝杆菌Erdman、H37Rv和H37Ra菌株以及牛分枝杆菌（BCG Tice）培养在Tween®-白蛋白、Proskauer和Beck或美国Trudeau学会媒体上。在美国特鲁多学会和普罗斯考尔和贝克培养基上培养的毒力生物接种剂对小鼠的中位致死剂量比在吐温白蛋白上培养的相应细菌低100到1000倍。这与器官计数技术测定的不同生物体的生长速率有关。由于培养基的作用，减毒菌几乎没有作用。对小鼠和豚鼠在Proskauer和Beck培养基上生长的生物体的毒力进行比较表明，皮下感染豚鼠的器官受累指数和静脉感染小鼠的肺、肝脏和脾脏的初始生长速度给出了相似的毒力评估。
Mycobacterium tuberculosis strains Erdman, H37Rv, and H37Ra, as well as M. bovis (BCG Tice), were grown on Tween®-albumin, Proskauer and Beck, or American Trudeau Society media. Inocula of the virulent organisms grown on American Trudeau Society and Proskauer and Beck media had a one hundred to one thousand fold lower median lethal dose for mice than the corresponding bacteria grown on Tween-albumin. This correlated with the growth rates determined for the different organisms by the organ enumeration technique. The attenuated organisms showed little or no effect ascribable to the culture medium. Comparisons of the virulence of organisms grown on Proskauer and Beck medium for mice and guinea pigs indicated that the organ involvement index in subcutaneously infected guinea pigs and the initial growth rate in the lungs, livers, and spleens of intravenously infected mice gave comparable assessments of virulence.