AUTOREGULATION AND NITRATE INHIBITION OF NODULE FORMATION IN SOYBEAN CV ENREI AND ITS NODULATION MUTANTS
AUTOREGULATION AND NITRATE INHIBITION OF NODULE FORMATION IN SOYBEAN CV ENREI AND ITS NODULATION MUTANTS
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DOI：
10.1093/jxb/44.3.547
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发表时间：
1993-03-01
影响因子：
6.9
通讯作者：
AKAO, S
中科院分区：
文献类型：
--
作者：
FRANCISCO, PB;AKAO, S
研究了大豆根瘤形成的调控和硝酸盐的抑制作用。我的天，进一步使用cv.恩丽非诱导突变体En115、En1282和En1314产生的显著卷曲根毛极少，且均无侵染线，且始终不能启动根皮层的表皮下细胞分裂（SCD）。在Enrei中，在结核出现之前发现了相当数量的被捕SCD，但在En6500中没有发现，En6500具有更多的SCD，在结核个体发育的更高级阶段逐渐增加。这些观察结果表明，自动调节作用，通过阻止结节出现之前的发育阶段。在Enrei和En6500中，出现的结节的成熟受到明显与自动调节无关的迟发性促凝控制机制的限制。在播种时接种的植物的相互楔形嫁接表明，控制的supercruculating表型驻留在地上部，而非cruculating表型是严格的根控制。目前的非突变突变体的突变表型的结果不是从改变的自动调节机制，但从突变，发挥根定位的影响，阻止SCD触发自动调节机制。在不同硝酸盐水平下生长的Enrei和En6500幼苗的相互嫁接实验表明，硝酸盐抑制植物生长，如自动调节，是拍摄控制。由于这两个过程总是一起表达，它们可能是因果关系，协同作用，以调节大豆根瘤的形成。这些结果表明，硝酸盐抑制和调节小麦叶片的硝酸盐积累是小麦叶片硝酸盐积累突变体的重要组成部分。与cv相似。布拉格突变体。
The regulation and nitrate inhibition of nodule formation in soybean, Glycine max (L.) Merr., was further examined using the nodulation mutants of cv. Enrei. The non-nodulating mutants En115, En1282, and En 1314 produced extremely few markedly-curled root hairs which were all devoid of infection threads, and invariably failed to initiate sub-epidermal cell divisions (SCDs) in the root cortex. A considerable number of arrested SCDs was found before nodule emergence in Enrei, but not in En6500 which had significantly more SCDs that progressively increased at more advanced stages of nodule ontogeny. These observations indicate that autoregulation acts by blocking the developmental stage before nodule emergence. In both Enrei and En6500, the maturation of emerged nodules was restricted by a late-acting nodulation control mechanism that is apparently unrelated to autoregulation. Reciprocal wedge-grafts of plants inoculated at sowing showed that the control of the supernodulating phenotype resides in the shoot, while the non-nodulating phenotype is strictly root-controlled. The nodulation phenotype of the current non-nodulating mutants results not from an alteration of the autoregulatory mechanism, but from mutation that exerts a root-localized effect that blocks SCDs which trigger the autoregulatory mechanism. Reciprocal grafting experiments on Enrei and En6500 seedlings grown under various nitrate levels suggest that nitrate inhibition of nodulation, like autoregulation, is shoot-controlled. Since these two processes are invariably expressed together, they are probably causally related, acting synergistically to regulate nodule formation in soybean. These results indicate that the regulation and nitrate inhibition of nodulation in the nodulation mutants of cv. Enrei are similar to those of cv. Bragg nodulation mutants.